TRANSPARENT TESTA GLABRA 1 ubiquitously regulates plant growth and development from Arabidopsis to foxtail millet (Setaria italica)
| Autor: | Chenhao Gao, Changyu Jin, Shuanghui Qi, Mingxun Chen, Baili Feng, Kaige Liu, Shaowei Duan, Dong Li |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Setaria Mutant Setaria Plant Arabidopsis Plant Development Plant Science 01 natural sciences 03 medical and health sciences Sequence Analysis Protein Stress Physiological Botany Tobacco Genetics Arabidopsis thaliana Cloning Molecular Phylogeny Nicotiana biology Arabidopsis Proteins Fatty Acids food and beverages General Medicine biology.organism_classification Plants Genetically Modified 030104 developmental biology Seedling Germination Seeds Ectopic expression Agronomy and Crop Science Sequence Alignment 010606 plant biology & botany Transcription Factors |
| Zdroj: | Plant science : an international journal of experimental plant biology. 254 |
| ISSN: | 1873-2259 |
| Popis: | TRANSPARENT TESTA GLABRA 1 of Arabidopsis thaliana (AtTTG1) is a WD40 repeat transcription factor that plays multiple roles in plant growth and development, particularly in seed metabolite production. In the present study, to determine whether SiTTG1 of the phylogenetically distant monocot foxtail millet (Setaria italica) has similar functions, we used transgenic Arabidopsis and Nicotiana systems to explore its activities. We found that SiTTG1 functions as a transcription factor. Overexpression of the SiTTG1 gene rescued many of the mutant phenotypes in Arabidopsis ttg1-13 plants. Additionally, SiTTG1 overexpression fully corrected the reduced expression of mucilage biosynthetic genes, and the induced expression of genes involved in accumulation of seed fatty acids and storage proteins in developing seeds of ttg1-13 plants. Ectopic expression of SiTTG1 restored the sensitivity of the ttg1-13 mutant to salinity and high glucose stresses during germination and seedling establishment, and restored altered expression levels of some stress-responsive genes in ttg1-13 seedlings to the wild type level under salinity and glucose stresses. Our results provide information that will be valuable for understanding the function of TTG1 from monocot to dicot species and identifying a promising target for genetic manipulation of foxtail millet to improve the amount of seed metabolites. |
| Databáze: | OpenAIRE |
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