Coffee induces AHR- and Nrf2-mediated transcription in intestinal epithelial cells
| Autor: | Linda M. P. Loonen, Prashanna Balaji Venkatasubramanian, Nicole de Wit, Jurriaan J. Mes, Jerry M. Wells, Gamze Toydemir |
|---|---|
| Přispěvatelé: | ALKÜ, 0-belirlenecek |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Antioxidant
Transcription Genetic Enterocyte NF-E2-Related Factor 2 medicine.medical_treatment AHR 01 natural sciences Coffee Nrf2 Analytical Chemistry 0404 agricultural biotechnology In vivo Transcription (biology) Caffeine medicine Cytochrome P-450 CYP1A1 Bioassay Humans Host-Microbe Interactomics Intestinal Mucosa Gene VLAG Food Health & Consumer Research Real-time qPCR Chemistry 010401 analytical chemistry Caco-2 04 agricultural and veterinary sciences General Medicine respiratory system 040401 food science Intestinal epithelium 0104 chemical sciences Cell biology Up-Regulation medicine.anatomical_structure Health & Consumer Research Receptors Aryl Hydrocarbon DR CALUX (R) DR CALUX® Food WIAS Caco-2 Cells Food Science |
| Zdroj: | Food Chemistry, 341(2) Food Chemistry 341 (2021) 2 |
| ISSN: | 0308-8146 |
| Popis: | WOS: 000602307500007 PubMed: 33038802 Coffee induces a health-promoting adaptive response of cells in the body. Here, we investigated enterocyte responses to AHR agonists in coffee and measured their transport across a polarized intestinal epithelium. AHR-activating potencies of Turkish, filter, and instant coffee were determined using DR CALUX (R) bioassay, before and after intestinal metabolization by Caco-2 cells. Furthermore, effects of coffee on induction of AHRand Nrf2-pathway genes in Caco-2 cells were evaluated by real-time qPCR. Coffee samples showed considerable AHRactivating potencies in DR CALUX (R) bioassay (up to 79% of positive control activity). After incubation with Caco2 cells, AHR activity of different coffees was between 35 and 64% of their initial value, suggesting rapid uptake and metabolization by epithelial cells. Expression of AHR-regulated gene CYP1A1 increased up to 41-fold and most Nrf2-pathway genes were up-regulated by coffee. This in vitro study may support the notion that coffee bioactives contribute to antioxidant defense and detoxification processes in vivo. Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); Dutch Ministry of Economic Affairs within the Systems Biology programme `Virtual Gut' [KB-17-003.02-021] The authors kindly thank Renata Ariens and Monic Tomassen for analytical support. This work was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK) and by the Dutch Ministry of Economic Affairs within the Systems Biology programme `Virtual Gut', KB-17-003.02-021. The funding sources were not involved in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication. |
| Databáze: | OpenAIRE |
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