Flavone ingredients can synergistically inhibit NDV infecting cell and improve ND vaccine's protective rate

Autor: Liu Xu, Liu Cui, Fan Yunpeng, Hu YuanLiang, Wang De-yun, Sun Zhan’ao, Yi Fang, Zhang Baokang, Liu Dan, Liu Jiaguo, Zhang Yu-qing
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Male
WDFF-BSRF-SF
wild dendranthema flower flavone plus baical skullcap root flavone plus sanchi flavone
Chick Embryo
Pharmacology
Antibodies
Viral
Biochemistry
MTT
3-(4
5-dimethylthiazol-2-yl)-2
5-diphenyltetrazolium bromide
Flavone ingredients prescriptions
WDFF-BSRF
wild dendranthema flower flavone plus baical skullcap root flavone
Structural Biology
CHMIs
Chinese herb medicinal ingredients
EF
epimedium flavone
BSRF
baical skullcap root flavone
Infectivity
chemistry.chemical_classification
Epimedium
EF-WDFF-BSRF
epimedium flavone plus wild dendranthema flower flavone plus baical skullcap root flavone
Antibody titer
ND
Newcastle disease
General Medicine
EF-BSRF
epimedium flavone plus baical skullcap root flavone
Protective rate
Titer
CHMs
Chinese herbal medicines
Antibody
HI
hemagglutination inhibition
medicine.drug_class
Newcastle Disease
WDFF
wild dendranthema flower flavone
PBS
phosphate-buffered saline
Newcastle disease virus
CMF
calcium and magnesium-free
Biology
EF-WDFF
epimedium flavone plus wild dendranthema flower flavone
BC
blank control
Flavones
Antiviral Agents
Virus
Article
MEM
minimum essential medium
Antivirus
medicine
SF
sanchi flavone
Animals
Mortality
Molecular Biology
Highest virus inhibitory rate
CEF
chicken embryo fibroblast
EF-WDFF-SF
epimedium flavone plus wild dendranthema flower flavone plus sanchi flavone
EF-BSRF-SF
epimedium flavone plus baical skullcap root flavone plus sanchi flavone
Plant Extracts
EF-SF-WDFF-BSRF
epimedium flavone plus sanchi flavone plus wild dendranthema flower flavone plus baical skullcap root flavone
DMSO
Dimethyl sulfoxide
Viral Vaccines
biology.organism_classification
MM
maintenance medium
EF-SF
epimedium flavone plus sanchi flavone
Virology
chemistry
NDV
Newcastle disease virus
VC
vaccine control
biology.protein
Antiviral drug
Morbidity
Chickens
Zdroj: International Journal of Biological Macromolecules
ISSN: 1879-0003
0141-8130
Popis: Highlights ► Flavone prescriptions composed with suitable compatibility could possess synergistical action of antiviral effect. ► EF-BSRF, EF-SF-WDFF-BSRF and EF-WDFF-BSRF could significantly inhibit the cellular infectivity of NDV in CEF. ► EF-SF-WDFF-BSRF could improve the protective effect of ND vaccine. ► EF-SF-WDFF-BSRF would be expected to exploit into a new-type antiviral drug.
In order to screen better flavone prescriptions of anti-Newcastle disease virus (NDV), four flavone ingredients of epimedium flavones (EF), baikal skullcap root flavones (BSRF), wild dendranthema flower flavones (WDFF), and sanchi flavones (SF) screened in previous experiments and their prescriptions were added into chicken embryo fibroblast monolayer with three drug-adding modes respectively. The cellular A570 values, the highest virus inhibitory rates and the score based on virus inhibitory rate were calculated to compare their antiviral activity. In immune protective test, the effects of three preparations (EF-BSRF, EF-SF-WDFF-BSRF and EF-WDFF-BSRF screened by the results in vitro experiment) on NDV infection were compared in chickens vaccinated with ND vaccine then challenged with NDV. Blood was regularly sampled for serum antibody titer determination. The pathogenic and dead statuses of chickens were clinically examined. The results indicated that the A570 values of the nine prescriptions, especially the foresaid three prescriptions at almost all concentrations in three drug-adding modes were significantly higher than that of the virus control group. The foresaid three prescriptions presented at the top five of the highest virus inhibitory rate, and located at the highest three of the score rank. The antibody titers and protective rates of the three prescriptions groups were higher than that of VC group, especially EF-SF-WDFF-BSRF group showed significant difference. These results indicated that flavone prescriptions composed with suitable compatibility could possess synergistical action of antiviral effect, ES-SF-WDFF-BSRF prescription could inhibit the cellular infectivity of NDV, improve the protective effect of ND vaccine and would be expected to exploit into a new-type antiviral drug.
Databáze: OpenAIRE
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