Detection and analysis of hepatitis C virus by a combined RT-PCR method: Variation in the 5' non-coding region of the viral genome
| Autor: | Spiros Linardopoulos, M. Ergazaki, Demetrios A. Spandidos, Andreas Karachristos |
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| Rok vydání: | 1995 |
| Předmět: |
Microbiology (medical)
Transcription Genetic Hepatitis C virus Molecular Sequence Data Oligonucleotides Genome Viral Hepacivirus Biology medicine.disease_cause Polymerase Chain Reaction Microbiology Virus law.invention Open Reading Frames Flaviviridae law Consensus Sequence medicine Humans Coding region Polymerase chain reaction DNA Primers Hepatitis Chronic Hepatitis Base Sequence medicine.diagnostic_test Genetic Variation General Medicine Oligonucleotides Antisense medicine.disease biology.organism_classification Hepatitis C Virology Real-time polymerase chain reaction Liver biopsy DNA Viral RNA Viral Sequence Alignment |
| Zdroj: | Journal of Medical Microbiology. 42:367-371 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/00222615-42-5-367 |
| Popis: | Surmmary A combined reverse transcription-polymerase chain reaction (RT-PCR) method was employed for the detection of hepatitis C virus (HCV) RNA in serum from patients with chronic active hepatitis, with primers corresponding to the 5′ non-coding region. The diagnosis was based on serological and biochemical methods and on liver biopsy. HCV-RNA was detected in 27 (90%) of 30 sera examined. The nucleotide sequence of PCR-amplified HCV cDNAs (256 bp) was determined from five specimens and heterogeneity varying between 0.58% and 2.89% among the clinical samples and the prototype HCV-1 was found. |
| Databáze: | OpenAIRE |
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