Comparison of ELISA for tissue transglutaminase autoantibodies with antiendomysium antibodies in pediatric and adult patients with celiac disease
| Autor: | A. Ocmant, S. Martino, Marcello Bagnasco, Vania Altrinetti, Paola Montagna, Giampaola Pesce, Desideria Descalzi, C. Salmaso, F. Mascart |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male congenital hereditary and neonatal diseases and abnormalities Adolescent Tissue transglutaminase Muscle Fibers Skeletal Immunology Child Welfare Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Asymptomatic Antibodies Coeliac disease Serology Immunopathology medicine Humans Immunology and Allergy Child Fluorescent Antibody Technique Indirect Aged Autoantibodies Aged 80 and over Transglutaminases biology business.industry Autoantibody Middle Aged Endomysium medicine.disease Celiac Disease medicine.anatomical_structure ROC Curve Child Preschool biology.protein Female Antibody medicine.symptom business |
| Zdroj: | Allergy. 56:544-547 |
| ISSN: | 1398-9995 0105-4538 |
| Popis: | Background: Tissue transglutaminase (t-TG) is the main autoantigen recognized by the endomysium antibodies (EMA) observed in patients with celiac disease (CD). The aim of the study was to assess an ELISA method for t-TG antibodies (t-TGA) with respect to EMA IF assay in pediatric and adult patients. Methods: t-TGA were analyzed by ELISA in 220 sera samples: 82 patients with biopsy-proven untreated CD (23 adults and 59 children), 14 CD children on gluten-free diet, 18 asymptomatic relatives of CD patients, and 106 age-matched control patients with gluten-unrelated gastrointestinal diseases (58 adults and 48 children). Serum IgA EMA were tested on umbilical cord sections in all patients. Results: The great majority (92.7%) of untreated CD patients (both adults and children) were t-TGA positive (values ranging from 20.1 to >300 AU). None of the child control patients and only two out of 58 (3.4%) of the adults with unrelated gastrointestinal diseases had serum t-TGA positivity; two out of 18 first-degree relatives with biopsy-proved silent CD were t-TGA (as well as EMA) positive. Finally, two out of 14 CD children, assuming a gluten-free diet, had serum t-TGA (as well as EMA). A highly significant correlation (P |
| Databáze: | OpenAIRE |
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