Component-resolved diagnosis with commercially availableD. pteronyssinusDer p 1, Der p 2 and Der p 10: relevant markers for house dust mite allergy
| Autor: | F. Porri, I. Deneux, C. Agabriel, V. Liabeuf, Pierre Bongrand, Denis Charpin, V. Grandné, Joana Vitte, M. Bronnert, Joëlle Birnbaum, Alexandre Fabre, Jean-Jacques Grob, Philippe Berbis, Isabelle Cleach, Julien Mancini |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Hypersensitivity Immediate Male Rhinitis Allergic Perennial Adolescent Dermatophagoides pteronyssinus Immunology Tropomyosin Immunoglobulin E Arthropod Proteins Dermatitis Atopic Young Adult Antigen medicine Animals Humans Immunology and Allergy Clinical significance Antigens Dermatophagoides Child Sensitization Aged Asthma Aged 80 and over House dust mite biology House dust mite allergy business.industry Pyroglyphidae Atopic dermatitis Middle Aged medicine.disease biology.organism_classification Cysteine Endopeptidases medicine.anatomical_structure Child Preschool Immunoglobulin G biology.protein Female Reagent Kits Diagnostic business |
| Zdroj: | Clinical & Experimental Allergy. 42:1406-1415 |
| ISSN: | 0954-7894 |
| DOI: | 10.1111/j.1365-2222.2012.04035.x |
| Popis: | Background Commercially available house dust mite components may improve routine testing of allergic patients. Objective To establish the prevalence and serum levels of IgE to commercial Der p 1, Der p 2, Der p 10 and the carbohydrate MUXF3 in house dust-mite allergic patients. To compare individual vs. allergen microarray methods. Methods Prevalence and serum levels of IgE to Dermatophagoides pteronyssinus extract and components Der p 1, Der p 2, Der p 10 and MUXF3, specific IgG4 to D. pteronyssinus, total serum IgE levels, and clinical features (age, asthma, rhinitis and atopic dermatitis) were determined in 123 patients (64 children) with the ImmunoCAP® method. ImmunoCAP ISAC® was performed in 24 patients. Results All patients had serum IgE to D. pteronyssinus. Prevalences of serum IgE to commercial components were Der p 1 93%, Der p 2 77% (Der p 1 or Der p 2 94%), Der p 10 28% and MUXF3 25%. Levels of D. pteronyssinus IgE strongly correlated with Der p 1 and Der p 2 IgE (r = 0.89 and 0.85 respectively), but not Der p 10 and MUXF3. ImmunoCAP® and ImmunoCAP ISAC® were concordant, but the quantitative correlation was poor. No clinical implication for the prevalence, levels, or molecular IgE reactivity profile to house dust mite components was found. Conclusions and Clinical Relevance Commercially available Der p 1 and Der p 2 strongly correlate with IgE D. pteronyssinus. The lack of Der p 1 and Der p 2 IgE may help with differential diagnosis. Der p 10 serum IgE prevalence and levels suggest different patterns in food and mite-related tropomyosin sensitization. Serum IgE to carbohydrate MUXF3, although unexpectedly prevalent, were low and did not modify D. pteronyssinus IgE levels. Follow-up may be best carried out with individual rather than microarrayed components. |
| Databáze: | OpenAIRE |
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