Corticotropin-Releasing Hormone and Its Structurally Related Urocortin Are Synthesized and Secreted by Human Mast Cells
| Autor: | Man Huang, Spyridon Christodoulou, Nikoletta Papadopoulou, Kristiana Kandere-Grzybowska, Achilles Athanassiou, B. Madhappan, Duraisamy Kempuraj, Michael Lytinas, William Boucher, Theoharis C. Theoharides |
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| Rok vydání: | 2004 |
| Předmět: |
endocrine system
medicine.medical_specialty Corticotropin-Releasing Hormone Gene Expression Enzyme-Linked Immunosorbent Assay Stimulation Vascular permeability Biology Proinflammatory cytokine Corticotropin-releasing hormone Endocrinology Immune system Stress Physiological Internal medicine medicine Humans Secretion Mast Cells RNA Messenger Cells Cultured Urocortins Urocortin Serine Endopeptidases Immunoglobulin E Fetal Blood Mast cell Immunohistochemistry medicine.anatomical_structure Tryptases hormones hormone substitutes and hormone antagonists |
| Zdroj: | Endocrinology. 145:43-48 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | Stress activates the hypothalamic-pituitary-adrenal axis through CRH, leading to production of glucocorticoids that down-regulate immune responses. However, acute stress also has proinflammatory effects. We previously showed that restraint stress, as well as CRH and its structurally related urocortin (Ucn), could activate mast cells and trigger mast cell-dependent vascular permeability. Here we show for the first time that human cord blood-derived cultured mast cells (hCBMC) at 10 wk, but not at 2 wk, are immunocytochemically positive for CRH and Ucn; human leukemic mast cells are weakly positive for both peptides. The ability of these mast cells to synthesize CRH and Ucn was confirmed by showing mRNA expression with RT-PCR. hCBMC (8–14 wk) synthesize and store 1–10 ng/106 cells (10–20 μg/g) of both CRH and Ucn detected by ELISA of cell homogenates. Stimulation of IgE-sensitized hCBMC with anti-IgE results in secretion of most CRH and Ucn. These findings indicate that mast cells are not only the target, but also a potential source of CRH and Ucn that could have both autocrine and paracrine functions, especially in allergic inflammatory disorders exacerbated by stress. |
| Databáze: | OpenAIRE |
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