Modified montmorillonite-bacterial cellulose composites as a novel dressing system for pressure injury
| Autor: | Rafael C. Dutra, Jaíne Ferrareis Menegasso, Tatiana Pineda Vásquez, Nayara Alves Celinca Moraes, Regina Vasconcellos Antônio, Francielly Andressa Felipetti |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Male
Wound Healing Skin wound Pressure injury Epidermis (botany) Scanning electron microscope Chemistry Positive control Hydrogels General Medicine Bandages Biochemistry Mice chemistry.chemical_compound Montmorillonite Structural Biology Bacterial cellulose mental disorders Bentonite Animals Fourier transform infrared spectroscopy Cellulose Molecular Biology Nuclear chemistry |
| Zdroj: | International Journal of Biological Macromolecules. 194:402-411 |
| ISSN: | 0141-8130 |
| Popis: | The main objective of this study was to investigate the effects of bacterial cellulose hydrogel (BCH) incorporated into montmorillonite (MMT) and its underlying mechanisms of action on a skin wound healing mouse model following pressure injury model. Komagataeibacter hansenii was used to obtain 5 cm in diameter and 0.8 mm of thickness circular bacterial cellulose (BC) sheets, which were incorporated with MMT by deposition ex-site using a 0.1% MMT suspension (100 rpm for 24 h at 28 °C). Afterward, Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM) were used to characterize the bacterial cellulose hydrogel incorporated into montmorillonite (BCH-MMT). The pressure injury model was assessed by macroscopic and histological analysis in male Swiss mice. Both, BC and BCH-MMT, showed a typical FTIR spectrum of cellulosic substrates with pronounces bands around 3344, 2920, 1637, and 1041 cm−1 while microparticles of MMT dispersed uniformly throughout BC were revealed by SEM photographs. Animals treated with BCH-MMT showed significant healing of pressure ulcers as demonstrated by reduced area of redness and spontaneous hyperalgesia, lower amounts of in-site inflammatory cells (to the same level as the positive control Dersani®) and ultimately, complete epidermis re-epithelialization and tissue regeneration. Altogether, these findings suggest that a modified BCH-MMT film could serve as scaffolding for skin tissue engineering and potentially as a novel dressing material for pressure injury. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect