Cloning of a potential cytochrome P450 from the archaeon Sulfolobus solfataricus
| Autor: | Robert H. White, Barbara T. Solow, Peter J. Kennelly, Kathryn Harris, Rhonda L. Wright |
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| Rok vydání: | 1996 |
| Předmět: |
Cytochrome
Evolution Archaebacteria ved/biology.organism_classification_rank.species Molecular Sequence Data Biophysics Cytochrome P450 Bacillus subtilis Biochemistry Polymerase Chain Reaction Conserved sequence Sulfolobus Evolution Molecular Cytochrome P-450 Enzyme System Structural Biology Genetics Amino Acid Sequence Cloning Molecular Molecular Biology Peptide sequence Gene Conserved Sequence Phylogeny chemistry.chemical_classification biology Base Sequence Sequence Homology Amino Acid ved/biology Sulfolobus solfataricus Cell Biology biology.organism_classification Archaea Amino acid body regions chemistry biology.protein CYP119 |
| Zdroj: | FEBS letters. 384(3) |
| ISSN: | 0014-5793 |
| Popis: | A gene, CYP119, for a potential cytochrome P450 has been isolated and sequenced from the extreme acidothermophilic archaeon Sulfolobus solfataricus. The gene predicts a polypeptide of 368 amino acids containing the consensus heme-binding sequence Phe-Gly-Xaa-Gly-Xaa-His-Xaa-Cys-Xaa-Gly- Xaa3-Ala-Arg-Xaa-Glu. It most closely resembles the cytochrome P450s found in the bacterium Bacillus subtilis, with which it shares 129 identical amino acid residues (35%). This first sequence of a potential archaeal cytochrome P450 represents an important step in tracing the complex evolutionary history of this biologically important enzyme family. |
| Databáze: | OpenAIRE |
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