Modular control of glutamatergic neuronal identity in C. elegans by distinct homeodomain proteins
| Autor: | Feifan Zhang, Richard J. Poole, Terry Felton, Estanisla Daniel De La Cruz, Oliver Hobert, Esther Serrano-Saiz |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Nervous system
Sensory Receptor Cells Regulator General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Glutamatergic Mice 0302 clinical medicine Vesicular Glutamate Transport Proteins medicine Animals Caenorhabditis elegans Caenorhabditis elegans Proteins 030304 developmental biology Homeodomain Proteins Neurons 0303 health sciences biology Biochemistry Genetics and Molecular Biology(all) Glutamate receptor Anatomy biology.organism_classification Phenotype medicine.anatomical_structure Receptors Glutamate Homeobox Neuron Neuroscience 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Cell. 155(3) |
| ISSN: | 1097-4172 |
| Popis: | The choice of using one of many possible neurotransmitter systems is a critical step in defining the identity of an individual neuron type. We show here that the key defining feature of glutamatergic neurons, the vesicular glutamate transporter EAT-4/VGLUT is expressed in 38 of the 118 anatomically defined neuron classes of the C.elegans nervous system. We show that eat-4/VGLUT expression is controlled in a modular manner, with distinct cis-regulatory modules driving expression in distinct glutamatergic neuron classes. We identify 13 different transcription factors, 11 of them homeodomain proteins, that act in specific combinations in 25 different glutamatergic neuron classes to initiate and maintain eat-4/VGLUT expression. We show that the adoption of a glutamatergic phenotype is linked to the adoption of other terminal identity features of a neuron, including cotransmitter phenotypes. Examination of mouse orthologs of these homeodomain proteins resulted in the identification of mouse LHX1 as a regulator of glutamatergic neurons in the brainstem. |
| Databáze: | OpenAIRE |
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