| Autor: |
Mao, Shiyu, Zhang, Wentao, Yang, Fuhan, Guo, Yadong, Wang, Hong, Wu, Yuan, Wang, Ruiliang, Maskey, Niraj, Zheng, Zongtai, Li, Cheng, Ma, Wenchao, Zhang, Junfeng, Yan, Yang, Yao, Xudong |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.16869880.v1 |
| Popis: |
Additional file 5: Figure S2. The circ_0004296 knockdown promoted the proliferation, migration, invasion, and EMT induction of PCa cells. A. Schematic illustration of designed siRNAs (siRNA#1, siRNA#2, and siRNA#3) targeting the back-splice junction of circ_0004296. B. The expression of circ_0004296 and ETS1 was detected by qRT-PCR in PC3 and DU145 cells transfected with siRNA-circ_0004296 or si-NC. C, D. CCK8 and colony formation assays were performed to measure the viability of PCa cells after circ_0004296 knockdown. E-H. The migration and invasion abilities of PCa cells were analyzed by wound healing and Transwell assays for migration and invasion after circ_0004296 knockdown. Scale bars =100um. I. Western blotting assay was performed to detected the expression of EMT-related proteins, namely E-cadherin, N-cadherin, Vimentin, and Snail, after circ_0004296 knockdown. J. Protein profiling was used to screen specific proteins bound by circ_0004296 in PCa cells. *P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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