Graphical approach to non-ideal cases of self-associating protein systems
Autor: | W. T. Cope, P. W. Chun, S. J. Kim, L. H. Tang, J. D. Williams, E. T. Adams |
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Rok vydání: | 1972 |
Předmět: | |
Zdroj: | Biopolymers. 11:197-214 |
ISSN: | 1097-0282 0006-3525 |
DOI: | 10.1002/bip.1972.360110115 |
Popis: | Our previous paper described graphical procedures for evaluating the mode of association in ideal discrete and indefinite cases. This paper concerns the application of such procedures in cases where the non-ideality term BM1 must be considered. Bovine liver L-glutamate dehydrogenase and lysozyme are used as model systems. Several graphical procedures for dealing with cases of 1 − m, 1 − m − n, or indefinite association are developed. The procedure is based on the evaluation of the weight-fraction monomer with-out prior calculation of BM1, using graphical analysis to evaluate the non-ideality term. |
Databáze: | OpenAIRE |
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