Graphical approach to non-ideal cases of self-associating protein systems

Autor: W. T. Cope, P. W. Chun, S. J. Kim, L. H. Tang, J. D. Williams, E. T. Adams
Rok vydání: 1972
Předmět:
Zdroj: Biopolymers. 11:197-214
ISSN: 1097-0282
0006-3525
DOI: 10.1002/bip.1972.360110115
Popis: Our previous paper described graphical procedures for evaluating the mode of association in ideal discrete and indefinite cases. This paper concerns the application of such procedures in cases where the non-ideality term BM1 must be considered. Bovine liver L-glutamate dehydrogenase and lysozyme are used as model systems. Several graphical procedures for dealing with cases of 1 − m, 1 − m − n, or indefinite association are developed. The procedure is based on the evaluation of the weight-fraction monomer with-out prior calculation of BM1, using graphical analysis to evaluate the non-ideality term.
Databáze: OpenAIRE