Fabrication of a BMP-2-immobilized porous microsphere modified by heparin for bone tissue engineering
| Autor: | Kyeongsoon Park, Sung-Wook Choi, Dong Hun Suh, Dong Hyup Shin, Young Pil Yun, Sungeun Kim, Kyu Sik Shim |
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| Rok vydání: | 2015 |
| Předmět: |
Scanning electron microscope
Nanotechnology Bone morphogenetic protein 2 Cell Line chemistry.chemical_compound Colloid and Surface Chemistry medicine Humans Osteopontin Physical and Theoretical Chemistry Bone regeneration Bone Development Tissue Engineering biology Gene Expression Profiling technology industry and agriculture Surfaces and Interfaces General Medicine Heparin Microspheres PLGA chemistry biology.protein Osteocalcin Alkaline phosphatase Biotechnology Biomedical engineering medicine.drug |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 134:453-460 |
| ISSN: | 0927-7765 |
| Popis: | The purpose of this study was to fabricate BMP-2-immobilized porous poly(lactide-co-glycolide) (PLGA) microspheres (PMS) modified with heparin for bone regeneration. A fluidic device was used to fabricate PMS and the fabricated PMS was modified with heparin-dopamine (Hep-DOPA). Bone morphogenic protein-2 (BMP-2) was immobilized on the heparinized PMS (Hep-PMS) via electrostatic interactions. Both PMS and modified PMS were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). MG-63 cell activity on PMS and modified PMS were assessed via alkaline phosphatase (ALP) activity, calcium deposition, and osteocalcin and osteopontin mRNA expression. Immobilized Hep-DOPA and BMP-2 on PMS were demonstrated by XPS analysis. BMP-2-immobilized Hep-PMS provided significantly higher ALP activity, calcium deposition, and osteocalcin and osteopontin mRNA expression compared to PMS alone. These results suggest that BMP-2-immobilized Hep-PMS effectively improves MG-63 cell activity. In conclusion, BMP-2-immobilized Hep-PMS can be used to effectively regenerate bone defects. |
| Databáze: | OpenAIRE |
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