Evaluation of miR-506 and miR-4316 expression in early and non-invasive diagnosis of colorectal cancer
Autor: | Dariusz M. Kowalski, Tomasz Powrózek, Janusz Milanowski, Joanna Dziwota, Paweł Krawczyk, Tomasz Olesiński, Adam Dmitruk |
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Rok vydání: | 2017 |
Předmět: |
Male
0301 basic medicine Oncology medicine.medical_specialty Colorectal cancer Short Communication Colonoscopy 03 medical and health sciences 0302 clinical medicine Internal medicine microRNA Early diagnosis and prevention medicine Humans Stage (cooking) Liquid biopsy Aged medicine.diagnostic_test business.industry Gastroenterology Hepatology medicine.disease Gene Expression Regulation Neoplastic Reverse transcription polymerase chain reaction MicroRNAs 030104 developmental biology ROC Curve 030220 oncology & carcinogenesis Biomarker (medicine) Female Colorectal Neoplasms business |
Zdroj: | International Journal of Colorectal Disease |
ISSN: | 1432-1262 0179-1958 |
Popis: | Purpose Examination of the entire colon by colonoscopy remains the golden standard for screening of colorectal cancer (CRC). However, patients are reluctant to perform invasive colonoscopies because of interference with their intimacy. Therefore, the potential use of non-invasive analysis of microRNAs expression in liquid biopsy as a novel biomarker for early CRC has investigated in several studies. In this study, we analyzed the expression of two novel microRNAs: miR-506 and miR-4316, which have never been examined in CRC. Methods Plasma samples were collected from 56 patients (median age of 68 years) with operable colorectal cancer and from 70 healthy individuals (median age of 59 years). Expression of plasma microRNAs was evaluated by quantitative reverse transcription polymerase chain reaction using Eco real-time PCR device (Illumina, USA). Results We found a significant elevated expression of both examined microRNAs in early CRC patients when compared to those in healthy individuals (p = 0.0054 for miR-506 and p = 0.0025 for miR-4316). The expression of miR-506 and miR-4316 did not depend on gender, age, disease stage, and tumor localization of CRC patients. ROC curve analysis showed that both examined microRNAs could differentiate early stage colorectal cancer from healthy individuals with 76.8% specificity and 60.7% sensitivity for miR-506 analysis and 76.8% specificity and 75% specificity for miR-4316 analysis. Conclusion Our study revealed that elevated expression of miR-506 and miR-4316 in peripheral blood were potential molecular markers for early colorectal cancer. |
Databáze: | OpenAIRE |
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