Angiogenesis without functional outcome after mononuclear stem cell transplant in a doxorubicin-induced dilated myocardiopathy murine model
| Autor: | Juan C. Chachques, Luiz Cesar Guarita-Souza, L. A. Rivetti, Mariester Malvezzi, Eltyeb Abdelwahid, W. Gremski, N. I. Myiague, Julio Cesar Francisco, Marcia Olandoski, L. Oliveira, Katherine Athayde Teixeira de Carvalho, Rossana Baggio Simeoni |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Pathology medicine.medical_specialty Angiogenesis medicine.medical_treatment Biomedical Engineering Medicine (miscellaneous) Neovascularization Physiologic Bioengineering 030204 cardiovascular system & hematology 01 natural sciences Transplantation Autologous Biomaterials Cell therapy 010104 statistics & probability 03 medical and health sciences 0302 clinical medicine Medicine Animals Doxorubicin Viability assay 0101 mathematics Rats Wistar Bone Marrow Transplantation Ejection fraction business.industry Bone Marrow Stem Cell General Medicine Stem-cell therapy Rats Disease Models Animal Stem cell business Cardiomyopathies medicine.drug Stem Cell Transplantation |
| Zdroj: | The International journal of artificial organs. 31(5) |
| ISSN: | 0391-3988 |
| Popis: | Objectives Cell transplantation is considered a novel approach in the treatment of myocardiopathy. The objective of this study was to evaluate the effects of autologous mononuclear stem cell therapy in doxorubicin-induced dilated myocardiopathy by conducting both functional and histopathologic analysis. Methods Seventy male rats were doxorubicin injected intraperitoneally for 2 weeks. At 1 month, the animals that had demonstrated left ventricular ejection fractions less than 40% were randomly divided into a mononuclear stem cell group and controls. Mononuclear stem cells were isolated. All animals underwent echocardiographic study: baseline, pre-cell therapy, and at 1 month post-cell therapy, and analyzed by the nonparametric Mann-Whitney test. Transplants were performed by subepicardial injections. Standard staining was performed. Results Twenty-three animals were randomly treated: mononuclear stem cell and control groups, with 11 rats completing the study. Cell viability was 85%. Mononuclear stem cells (n=5; 5×106 cells /300 μL medium) and control (n=6; 300 μL medium) were used. The resulting left ventricular ejection fraction in the cell therapy group was not significantly different compared with controls (p=0.54). New vessels were demonstrated in the subepicardial region. Conclusions Autologous mononuclear stem cell therapy was not functionally effective in doxorubicin-induced dilated myocardiopathy in the animal model under study with the experimental conditions, despite occurrence of angiogenic activity. |
| Databáze: | OpenAIRE |
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