A novel understanding of global DNA methylation in bobcat (Lynx rufus)
| Autor: | Brenda M. Murdoch, Stephanie D. McKay, Stefano Frattini, Hannah Lachance, Andrea Talenti, Sydney R. Friedman, Bonnie Cantrell, Chris Bernier, Paola Crepaldi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Felidae Range (biology) Zoology Conservation Wildlife Biology 010603 evolutionary biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Carcass weight Genetics Epigenetics Molecular Biology General Medicine 030104 developmental biology chemistry DNA methylation Fish Differential Methylation Conservation biology DNA Biotechnology |
| Zdroj: | Cantrell, B, Friedman, S, Lachance, H, Bernier, C, Murdoch, B, Frattini, S, Talenti, A, Crepaldi, P & McKay, S 2019, ' A novel understanding of global DNA methylation in bobcat (Lynx rufus) ', Genome, pp. 1-6 . https://doi.org/10.1139/gen-2019-0046 |
| ISSN: | 1480-3321 0831-2796 |
| Popis: | Epigenetic mechanisms may provide a novel prospective of bobcat (Lynx rufus) adaptation to habitat loss/fragmentation. Previous research has focused on bobcat behavior and genetics, but epigenetics has not been studied in bobcat. The aim of this study was to determine the quantity of global DNA methylation in the liver of 30 bobcats. DNA was extracted from liver samples obtained from the Vermont Fish and Wildlife Department. The percent of global DNA methylation was quantified and calculated using the MethylFlashTM Methylated DNA 5-mC Quantification Kit from Epigentek (Farmingdale, NY, USA). Age, sex, and carcass weight data were collected at sampling and analyzed with percent of global DNA methylation. Global DNA methylation was found to range from 0.46% to 2.76%. Age ranged from |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|