Activation of murine macrophages by Neisseria meningitidis and IFN-gamma in vitro: distinct roles of class A scavenger and Toll-like pattern recognition receptors in selective modulation of surface phenotype
| Autor: | Siamon Gordon, Leanne Peiser, Subhankar Mukhopadhyay |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
CD36 Antigens
Lipopolysaccharides Immunology Receptors Cell Surface In Vitro Techniques Neisseria meningitidis Biology Interferon-gamma Mice Antigens CD medicine Animals Immunology and Allergy Lectins C-Type Receptors Immunologic Scavenger receptor Receptor Mice Knockout CD86 Membrane Glycoproteins Innate immune system Tumor Necrosis Factor-alpha Macrophages Toll-Like Receptors Histocompatibility Antigens Class II Pattern recognition receptor Scavenger Receptors Class A Cell Biology T helper cell Acquired immune system Immunity Innate Up-Regulation Cell biology Toll-Like Receptor 4 Chemotaxis Leukocyte Mannose-Binding Lectins Phenotype medicine.anatomical_structure B7-1 Antigen B7-2 Antigen Inflammation Mediators Mannose Receptor Mannose receptor |
| Popis: | Innate and adaptive immune activation of macrophages (Mφ) by microorganisms and antigen-activated lymphoid cells, respectively, plays an important role in host defense and immunopathology. Antigen-presenting cells express a range of pattern recognition receptors including the class A types I and II scavenger receptors (SR-A) and Toll-like receptors (TLR). Recognition of microbial products by SR-A and TLR controls uptake, killing, altered gene expression, and the adaptive immune response; however, the contribution of each receptor and interplay with cytokine stimuli such as interferon-γ (IFN-γ) are not defined. We used Neisseria meningitidis (NM), a potent activator of innate immunity, and IFN-γ, a protoypic T helper cell type 1 proinflammatory cytokine, to compare surface antigens, secretion of mediators, and receptor functions in elicited peritoneal Mφ from wild-type and genetically modified mouse strains. We show that these stimuli regulate major histocompatibility complex type II (MHC-II) and costimulatory molecules differentially, as well as expression of the mannose receptor and of Mφ receptor with collagenous structure (MARCO), a distinct SR-A, which provides a selective marker for innate activation. In combination, NM inhibited up-regulation of MHC-II by IFN-γ while priming enhanced release of tumor necrosis factor α and nitric oxide. The SR-A contributes to phagocytosis of the organisms but not to their ability to induce CD80, CD86, and MARCO or to inhibit MHC-II. Conversely, studies with lipopolysaccharide (LPS)-deficient organisms and/or TLR-4 mutant mice showed that LPS and TLR-4 are at least partially required to induce CD80, CD86, and MARCO, but LPS is not required to inhibit MHC-II. These studies provide an experimental model and identify surface markers for analysis of innate and acquired immune activation of Mφ. |
| Databáze: | OpenAIRE |
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