Efficacy of Particulate Amniotic Membrane and Umbilical Cord Tissues in Attenuating Cartilage Destruction in an Osteoarthritis Model
| Autor: | Mei-Shu Shih, Julie O'Connell, Andrew L. Raines, Chen-Wei Su, Scheffer C.G. Tseng, Lorraine Chua |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Biomedical Engineering Bioengineering Osteoarthritis Matrix (biology) Biochemistry Umbilical cord Umbilical Cord Biomaterials 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Cartilage destruction Amnion Cartilage degeneration 030203 arthritis & rheumatology Saline control business.industry X-Ray Microtomography Osteoarthritis Knee medicine.disease Rats Cartilage 030104 developmental biology Membrane medicine.anatomical_structure Rats Inbred Lew Female business Medial meniscus |
| Zdroj: | Tissue Engineering Part A. 23:12-19 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2016.0088 |
| Popis: | Osteoarthritis (OA) is a progressive degenerative joint disease, and to date, no disease-modifying OA drug exists. Amniotic membrane and umbilical cord products have been used clinically in several diseases due to their anti-inflammatory and antiscarring properties. In the present study, we sought to evaluate whether a particulate amniotic membrane and umbilical cord (AM/UC) matrix could aid in attenuating disease progression. Lewis rats underwent medial meniscus transection (MMT) to induce OA. Two weeks after surgery, animals received intra-articular injections (50 μL) of either 50 or 100 μg/μL particulate AM/UC or saline control and were subsequently euthanized 1 or 4 weeks later. Cartilage degeneration was assessed using both histological scoring methods and equilibrium partitioning of an ionic contrast agent-microcomputed tomography (EPIC-μCT). EPIC-μCT analysis demonstrated that overall cartilage destruction was attenuated, with a significant increase in both cartilage thickness and volume as well as a significant decrease in total lesion area in animals injected with either dose of particulate AM/UC at 1 week, but only a high dose at 4 weeks postinjection. Osteoarthritis Research Society International (OARSI) histology scores of tibial sections corroborated EPIC-μCT results. Overall joint destruction was attenuated in animals injected with either dose of AM/UC tissue compared with saline-injected control animals at 1 week postinjection. Only high-dose AM/UC-injected animals continued to show less overall joint destruction by 4 weeks postinjection. Intra-articular injection of particulate AM/UC tissue attenuates cartilage degradation in a rat MMT model of OA, suggesting that it may be able to slow joint destruction in patients with OA. |
| Databáze: | OpenAIRE |
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