Purification and partial characterization of Cu/Zn superoxide dismutase from Kluyveromyces marxianus yeast
| Autor: | Trayana Nedeva, Vesela Moshtanska, V.Y. Petrova, Anna Kujumdzieva, Wolfgang Voelter, Pavlina Dolashka-Angelova |
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| Rok vydání: | 2009 |
| Předmět: |
Clinical Biochemistry
Biochemistry Analytical Chemistry Fungal Proteins Superoxide dismutase Kluyveromyces Kluyveromyces marxianus Enzyme Stability Glycoproteins Thermostability chemistry.chemical_classification Fungal protein Chromatography biology Superoxide Dismutase Chromatofocusing Temperature Cell Biology General Medicine Hydrogen-Ion Concentration Chromatography Ion Exchange biology.organism_classification Yeast Enzyme chemistry biology.protein |
| Zdroj: | Journal of Chromatography B. 877:3529-3536 |
| ISSN: | 1570-0232 |
| Popis: | A new thermostable Cu/Zn SOD from a thermotolerant yeast strain Kluyveromyces marxianus NBIMCC 1984 has been purified and characterized. The purification procedure comprises thermal treatment and dialysis, ion-exchange chromatography and chromatofocusing. The methodology is a rapid, efficient and highly specific, generating pure preparation (specific activity 996 U mg of protein(-1)) with a yield of 53%. The purified enzyme is a homodimer with Mw of 34,034 Da and has high N-terminal homology with other yeasts' Cu/Zn SOD enzymes. The protein is characterized with some unique features such as-thermostability (t(1/2) at 70 degrees C=30 min), pH stability in the alkaline range (7.5-8.5) and resistance to inhibitors and variety of chemicals. These characteristics reveal possibilities for wide practical application of K. marxianus Cu/Zn SOD enzyme. |
| Databáze: | OpenAIRE |
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