Synthetic Human Parathyroid Hormone Fragment Stimulates Prostaglandin E2 Synthesis by Chick Calvariae*
| Autor: | C. Y. Yang, Peter Polgar, W. A. Gonnerman, R. B. Nimberg, Linda Taylor |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
medicine.medical_specialty
Indomethacin Prostaglandin Endogeny Stimulation 6-Ketoprostaglandin F1 alpha Dinoprost Bone and Bones Dinoprostone Bone remodeling chemistry.chemical_compound Organ Culture Techniques Endocrinology Teriparatide Internal medicine Bone cell medicine Animals Bone Resorption Prostaglandin E2 Hyperparathyroidism Prostaglandins E Prostaglandins F medicine.disease Hormones Peptide Fragments Thromboxane B2 chemistry Parathyroid Hormone Cats lipids (amino acids peptides and proteins) Arachidonic acid medicine.drug |
| Zdroj: | Endocrinology. 120:63-70 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | Synthetic human PTH N-terminal 1-34 peptide [hPTH-(l-34)] stimulates prostaglandin (PG) production by chick calvariae in culture. PGE2 was the predominant PG found by both RIA and HPLC. Stimulation of PGE2 synthesis was significant at 50 ng/ml (1.2 × 10-8 M) hPTH-(l-34) and was dose dependent at concentrations up to 0.6 μg/ml (1.4 × 10-7 M). Continuous exposure of calvariae to hPTH-(l-34) showed that PGE2 production increased significantly by 1 h, reached a maximum at 24 h, and then persisted over 96 h. Indomethacin at concentrations above 5 × 10-7 M inhibited PGE2 synthesis by both control and hPTH-(l-34)-treated bones. PTH-(l-34) stimulated bone cells to convert arachidonic acid to PGE2, but did not activate the bone to release stored arachidonate. In summary, our results show that hPTH-(l-34) stimulates PGE2 synthesis by chick calvariae. This endogenous PGE2 may be involved in bone remodeling. (Endocrinology 120: 63–70,1987) |
| Databáze: | OpenAIRE |
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