Gut barrier impairment by high-fat diet in mice depends on housing conditions
| Autor: | Hannelore Daniel, Jarlei Fiamoncini, Thomas Clavel, Dirk Haller, Tamara Zietek, Ilias Lagkouvardos, Veronika Maria Müller, Florian Rohm |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Panniculitis Mice Obese Inflammation Biology Gut flora Diet High-Fat Bile Acids and Salts 03 medical and health sciences Feces Internal medicine medicine Animals Large intestine Obesity Barrier function Tight Junction Proteins Gut barrier Ussing chamber biology.organism_classification medicine.disease Housing Animal Endotoxemia Gastrointestinal Microbiome Intestines Mice Inbred C57BL 030104 developmental biology Endocrinology medicine.anatomical_structure Immunology medicine.symptom Food Science Biotechnology |
| Zdroj: | Molecular nutritionfood research. 60(4) |
| ISSN: | 1613-4133 |
| Popis: | cope Diet-induced obesity (DIO) is proposed to cause impairments in intestinal barrier integrity, but contradictory results have been published and it appears that the outcomes depend on other environmental factors. We therefore assessed whether the hygienic status of animal facilities alters the gut barrier in DIO mice. Methods and results Male C57BL/6N mice were housed in a conventional (CV) or a specific pathogen-free (SPF) animal facility and were fed identical diets represented by a high-fat (60kJ% fat) or control diet (11kJ% fat) for 12 wks. Intestinal barrier function in small and large intestine was evaluated in Ussing chambers by electrical resistance and permeability measurements. Jejunal (p < 0.01) and proximal colonic (p < 0.05) barrier function was altered in CV DIO mice, but not in SPF DIO mice. Moreover, only CV DIO mice were characterized by metabolic endotoxemia and low-grade inflammation. High-throughput 16S rRNA gene sequencing revealed significant differences in fecal bacterial diversity and composition between the two animal facilities, but only in mice fed the HFD. Moreover, cecal DCA concentrations correlated positively with two yet uncultivated Clostridiales species. Conclusions We demonstrated that housing conditions and associated changes in gut bacterial colonization are pivotal for maintenance of gut barrier integrity in DIO mice. |
| Databáze: | OpenAIRE |
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