Combination of MPPa-PDT and HSV1-TK/GCV gene therapy on prostate cancer
| Autor: | Liming Liang, Yuanyuan Tian, Wenxiang Bi, Weiwen Chen, Yani Lin |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Ganciclovir Cell Survival medicine.medical_treatment Genetic enhancement Apoptosis Photodynamic therapy Herpesvirus 1 Human Dermatology Transfection Thymidine Kinase Flow cytometry 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Animals Humans Cytotoxicity Endoplasmic Reticulum Chaperone BiP Expression vector medicine.diagnostic_test Chemistry Fatty Acids Prostatic Neoplasms Genetic Therapy Combined Modality Therapy Molecular biology 030104 developmental biology Photochemotherapy Thymidine kinase 030220 oncology & carcinogenesis Surgery medicine.drug |
| Zdroj: | Lasers in Medical Science. 33:227-232 |
| ISSN: | 1435-604X 0268-8921 |
| Popis: | We combined pyropheophorbide-a methyl ester, photodynamic therapy (MPPa-PDT), 670 ± 10 nm, 4 mW/cm2, with herpes simplex virus type 1 thymidine kinase/ganciclovir (HSV1-TK/GCV) to improve the therapeutic effect. We built HSV1-TK expression vector GV230-TK and we observed a bright green fluorescence under fluorescence microscope. It indicated the recombinant plasmid was transfected into PC-3M prostate cancer cells successfully. As the abundant glucose-regulated protein 78 (GRP78) promoter in PC-3M cells can cause active expression of HSV1-TK, cell protein was collected for western blot to determine the expression of HSV1-TK. In CCK-8 assay (n = 6), the cell survival rate of combined treatment group was about 10%, less than that of pure MPPa-PDT group (23%) and pure HSV1-TK/GCV group (35%) (t test, P |
| Databáze: | OpenAIRE |
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