Grape ASR-Silencing Sways Nuclear Proteome, Histone Marks and Interplay of Intrinsically Disordered Proteins
| Autor: | Atanassov, Hristo, Parrilla, Jonathan, Artault, Caroline, Verbeke, Jérémy, Schneider, Thomas, Grossmann, Jonas, Roschitzki, Bernd, Atanassova, Rossitza |
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| Přispěvatelé: | University of Zurich, Atanassova, Rossitza |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Proteomics
1503 Catalysis QH301-705.5 1607 Spectroscopy 610 Medicine & health 10071 Functional Genomics Center Zurich Catalysis Cell Line Epigenesis Genetic Inorganic Chemistry Histones ASR Gene Expression Regulation Plant LEA D-29 1312 Molecular Biology 1706 Computer Science Applications Vitis Physical and Theoretical Chemistry grape embryogenic cells histone PTMs IDPs iTRAQ nuclear proteome VvMSA-RNAi silencing Biology (General) Molecular Biology QD1-999 Spectroscopy Cell Nucleus 1604 Inorganic Chemistry Organic Chemistry fungi General Medicine Computer Science Applications Histone Code Intrinsically Disordered Proteins Chemistry 10036 Medical Clinic Gene Knockdown Techniques 570 Life sciences biology 1606 Physical and Theoretical Chemistry Protein Processing Post-Translational 1605 Organic Chemistry Abscisic Acid |
| Zdroj: | International Journal of Molecular Sciences, Vol 23, Iss 1537, p 1537 (2022) International Journal of Molecular Sciences, 23 (3) International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1537 |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | In order to unravel the functions of ASR (Abscisic acid, Stress, Ripening-induced) proteins in the nucleus, we created a new model of genetically transformed grape embryogenic cells by RNAi-knockdown of grape ASR (VvMSA). Nuclear proteomes of wild-type and VvMSA-RNAi grape cell lines were analyzed by quantitative isobaric tagging (iTRAQ 8-plex). The most significantly up-or down-regulated nuclear proteins were involved in epigenetic regulation, DNA replication/repair, transcription, mRNA splicing/stability/editing, rRNA processing/biogenesis, metabolism, cell division/differentiation and stress responses. The spectacular up-regulation in VvMSA-silenced cells was that of the stress response protein VvLEA D-29 (Late Embryogenesis Abundant). Both VvMSA and VvLEA D-29 genes displayed strong and contrasted responsiveness to auxin depletion, repression of VvMSA and induction of VvLEA D-29. In silico analysis of VvMSA and VvLEA D-29 proteins highlighted their intrinsically disordered nature and possible compensatory relationship. Semi-quantitative evaluation by medium-throughput immunoblotting of eighteen post-translational modifications of histones H3 and H4 in VvMSA-knockdown cells showed significant enrichment/depletion of the histone marks H3K4me1, H3K4me3, H3K9me1, H3K9me2, H3K36me2, H3K36me3 and H4K16ac. We demonstrate that grape ASR repression differentially affects members of complex nucleoprotein structures and may not only act as molecular chaperone/transcription factor, but also participates in plant responses to developmental and environmental cues through epigenetic mechanisms. International Journal of Molecular Sciences, 23 (3) ISSN:1422-0067 |
| Databáze: | OpenAIRE |
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