Microvascular Pericytes Express Aggrecan Message Which Is Regulated by BMP-2
| Autor: | David L. Diefenderfer, Carl T. Brighton |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Bone sialoprotein
Biophysics Bone Morphogenetic Protein 2 Gene Expression Biochemistry Bone morphogenetic protein 2 Versicans Transforming Growth Factor beta medicine Animals Lectins C-Type Aggrecans RNA Messenger Molecular Biology Endochondral ossification Cells Cultured Aggrecan DNA Primers Fracture Healing Extracellular Matrix Proteins Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction Microcirculation Mesenchymal stem cell Cell Biology Molecular biology Recombinant Proteins Phenotype medicine.anatomical_structure Chondroitin Sulfate Proteoglycans Bone Morphogenetic Proteins biology.protein Versican Cattle Proteoglycans Collagen Pericyte Pericytes Chondrogenesis Type I collagen |
| Zdroj: | Biochemical and Biophysical Research Communications. 269:172-178 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2000.2148 |
| Popis: | Multipotential mesenchymal stem cells capable of chondro-osseous induction contribute to the endochondral callus of healing fractured bone. Microvascular pericytes serving the role of multipotential mesenchymal stem cells are considered osteoprogenitors because they express type I collagen, alkaline phosphatase enzyme activity, osteocalcin immunoreactivity, and bone sialoprotein mRNA. Previous electron microscopic studies indicate that this cell type has a contribution to the fracture callus. Limited data suggest that pericytes may also assume a chondrogenic phenotype. We undertook in vitro studies to understand how the chondro-osseous phenotype of the pericyte might be regulated. Using Northern analysis and semiquantitative reverse transcriptase–polymerase chain reaction (RT-PCR), we found that cultured pericytes produce aggrecan and type II collagen mRNA indicating their chondrogenic potential. Aggrecan message is elevated by BMP-2 as analyzed by both Northern hybridization and RT-PCR. This finding suggests a regulatory role for this morphogen on this phenotype in pericytes. RT-PCR amplified versican product was also associated with pericyte cultures but was not affected by BMP-2. Our data strongly support a chondrogenic role for the pericyte and that the phenotype is regulated at least in part by BMP. |
| Databáze: | OpenAIRE |
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