Tea polyphenols induced apoptosis of breast cancer cells by suppressing the expression of Survivin
| Autor: | Xiaoqun Dong, Li Cai, Guoquan Cui, Hong Sun, Qiushi Lin, Yan Wang, Jian Wang, Xuesong Chen, Yu Li |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty Cyclin E Survivin Cell Mice Nude Apoptosis Breast Neoplasms Article Inhibitor of Apoptosis Proteins Mice Cyclin D1 Cell Line Tumor Proliferating Cell Nuclear Antigen medicine Animals Humans Cell Proliferation Cell Nucleus Multidisciplinary Dose-Response Relationship Drug Tea biology Cell growth business.industry Polyphenols Apoptotic body Antineoplastic Agents Phytogenic Xenograft Model Antitumor Assays Mitochondria Tumor Burden Proliferating cell nuclear antigen Gene Expression Regulation Neoplastic medicine.anatomical_structure MCF-7 Cells Cancer research biology.protein Female business Signal Transduction |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep04416 |
| Popis: | To study the mechanism of tea polyphenols (TP)-induced apoptosis of breast cancer cells. Proliferation of MCF-7 and SK-BR-3 cells was evaluated by MTT assays. Cellular ultrastructure was examined by electron microscopy. Apoptosis was detected by TUNEL. PCNA、 Cyclin D1、 Cyclin E and Survivin expression was measured by Western blot. Cell proliferation was significantly inhibited by TP. Spindle and round cells were loosely distributed with increased particles after TP treatment. Increased cell size, frequent nuclear atypia and a collapse of apoptosis were observed. The nucleus was pushed towards one side, while the cytoplasm was rich in free ribosome. The membrane of mitochondria was thickening, and the cell apoptotic body was observed. TP treated cells experienced significantly enhanced apoptosis compared with 5-Fu treated or control groups. The expression of survivin was downregulated by TP. To conclude, TP can inhibit cell growth and induce apoptosis through downregulating the expression of survivin in breast cancer. |
| Databáze: | OpenAIRE |
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