Proximal upstream region of zebrafish bone morphogenetic protein 4 promoter directs heart expression of green fluorescent protein
| Autor: | Hsuan Shentu, Chang Jen Huang, Guor Mour Her, Sheng-Ping L. Hwang, Hui Ju Wen, Jen-Leih Wu |
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| Rok vydání: | 2003 |
| Předmět: |
animal structures
Transgene Green Fluorescent Proteins Embryonic Development Endogeny Bone Morphogenetic Protein 4 Biology Green fluorescent protein chemistry.chemical_compound Endocrinology Genetics Animals Promoter Regions Genetic Zebrafish Intron Gene Expression Regulation Developmental Embryo Heart Cell Biology Zebrafish Proteins biology.organism_classification Molecular biology Luminescent Proteins Bone morphogenetic protein 4 chemistry embryonic structures Bone Morphogenetic Proteins Indicators and Reagents DNA |
| Zdroj: | Genesis (New York, N.Y. : 2000). 37(3) |
| ISSN: | 1526-954X |
| Popis: | We examined the activity of the bone morphogenetic protein 4 (BMP4) promoter in zebrafish embryos via transient and stable transgenic expression analyses in order to obtain a better understanding of the regulation of BMP4 tissue-specific expression. Transient expression studies showed that the 9.0-kb BMP4 promoter/upstream region drove green fluorescent protein (GFP) expression mainly in the heart. Deletion analyses indicated the existence of multiple regulatory elements in the 7.5-kb BMP4 promoter/proximal upstream region. In addition, a coinjection experiment further demonstrated the 2.4-kb Bgl II–Hind III DNA region contains major positive regulatory elements. In addition, stable transgenic lines were established to further confirm the heart-specificity of this segment in BMP4 promoter. The results showed that GFP was mainly localized in the myocardium of developing ventricles of 48-hpf (hours postfertilization), 72-hpf, and 100-hpf transgenic F1 embryos. Together, these results indicate that the 7.5-kb BMP4 promoter/proximal upstream region specifically contains regulatory elements for BMP4 expression in the heart, while regulatory elements for other endogenous BMP4-expressing tissues may reside in more distal regions and/or in introns. genesis 37:103–112, 2003. © 2003 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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