De Novo Genome Assembly of the Meadow Brown Butterfly, Maniola jurtina
| Autor: | David J. Hosken, Manmohan Sharma, Nina Wedell, Chris Bass, Konrad Paszkiewicz, Richard H. ffrench-Constant, Simon W. Baxter, Kumar Saurabh Singh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Sequence assembly
comparative genomics Biology QH426-470 Genome 03 medical and health sciences 0302 clinical medicine Genotype Genetics maniola jurtina Molecular Biology Gene Genetics (clinical) 030304 developmental biology meadow brown Comparative genomics Whole genome sequencing 0303 health sciences biology.organism_classification Phenotype Genome Report Meadow brown Evolutionary biology Minion genome assembly lepidoptera 030217 neurology & neurosurgery |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 10, Iss 5, Pp 1477-1484 (2020) G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.120.401071 |
| Popis: | BackgroundMeadow brown butterflies (Maniola jurtina) on the Isles of Scilly represent an ideal model in which to dissect the links between genotype, phenotype and long-term patterns of selection in the wild - a largely unfulfilled but fundamental aim of modern biology. To meet this aim, a clear description of genotype is required.FindingsHere we present the draft genome sequence of M. jurtina to serve as an initial genetic resource for this species. Seven libraries were constructed using DNA from multiple wild caught females and sequenced using Illumina, PacBio RSII and MinION technology. A novel hybrid assembly approach was employed to generate a final assembly with an N50 of 214 kb (longest scaffold 2.9 Mb). The genome encodes a total of 36,294 genes. 90.3% and 88.7% of core BUSCO (Benchmarking Universal Single-Copy Orthologs) Arthropoda and Insecta gene sets were recovered as complete single-copies from this assembly. Comparisons with 17 other Lepidopteran species placed 86.5% of the assembled genes in orthogroups.ConclusionsOur results provide the first high-quality draft genome and annotation of the butterfly M. jurtina. |
| Databáze: | OpenAIRE |
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