Phyllolobium chinense Fisch Flavonoids (PCFF) Suppresses the M1 Polarization of LPS-Stimulated RAW264.7 Macrophages by Inhibiting NF-κB/iNOS Signaling Pathway
| Autor: | Xu Wang, Xu-Dong Hu, Du Li, Hua Fan, Qiong Wu, Yidan Dong, Ping Liu, Longping Peng, Youhua Wang, Min Cao |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
p38 mitogen-activated protein kinases Inflammation nuclear factor-κB signaling pathway Nitric oxide 03 medical and health sciences chemistry.chemical_compound Phyllolobium chinense Fisch flavonoids (PCFF) 0302 clinical medicine Gene expression medicine Macrophage Pharmacology (medical) Zebrafish Original Research Pharmacology inducible nitric oxide synthases biology lcsh:RM1-950 NF-κB inflammatory response zebrafish biology.organism_classification Cell biology lcsh:Therapeutics. Pharmacology 030104 developmental biology chemistry 030220 oncology & carcinogenesis Signal transduction medicine.symptom RAW264.7 macrophages |
| Zdroj: | Frontiers in Pharmacology Frontiers in Pharmacology, Vol 11 (2020) |
| ISSN: | 1663-9812 |
| DOI: | 10.3389/fphar.2020.00864 |
| Popis: | Background M1 macrophage plays an important role in inflammatory reaction. In this study, potential anti-inflammatory effect of Phyllolobium chinense Fisch flavonoids (PCFF) was assessed via Zebrafish acute inflammation model in vivo and LPS-induced pro-inflammatory M1 macrophage model in vitro. Methods The quality control of P. chinense Fisch flavonoids (PCFF) was analyzed by HPLC. Anti-inflammatory effect of PCFF on the acute injured zebrafish was evaluated by the migration of fluorescence labeled macrophages and neutrophils, and the gene expression of inflammatory factors. In addition, the anti-inflammatory mechanism of PCFF was investigated by the related gene expression and related signaling pathway regulation of pro-inflammatory mediators in LPS-induced pro-inflammatory M1 RAW264.7 macrophage. Results P. chinense Fisch flavonoids (PCFF) markedly suppressed macrophage and neutrophil migration and iNOS gene expression in acute injured zebrafish with tail-cutting. PCFF significantly inhibited NO overproduction and iNOS gene overexpression in LPS-sitimulated pro-inflammatory M1 RAW264.7 macrophages. What's more, PCFF could evidently decrease p65 protein production, but had no effect on the production of P38, JNK and ERK1/2 proteins. Conclusion P. chinense Fisch flavonoids (PCFF) have a remarkable inhibitory effect on the inflammatory response in acute injured zebrafish and LPS-stimulated M1 RAW264.7 macrophage. The pharmacological mechanism may be related to the regulation of NO overproduction and the inhibition of NF-κB/iNOS signaling pathway. |
| Databáze: | OpenAIRE |
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