Therapeutic Effects against Tissue Necrosis of Remote Ischemic Preconditioning Combined with Human Adipose-Derived Stem Cells in Random-Pattern Skin Flap Rat Models
| Autor: | Soo Young Moon, Hyo Jin Kang, Chang Sik Pak, Young Eun Lee |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty Necrosis Angiogenesis Skin flap Adipose tissue Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Random pattern medicine Animals Humans cardiovascular diseases Ischemic Preconditioning business.industry Stem Cells Therapeutic effect Rats 030220 oncology & carcinogenesis Reperfusion Injury Tissue necrosis Ischemic preconditioning 030211 gastroenterology & hepatology Surgery Female medicine.symptom business |
| Zdroj: | Journal of investigative surgery : the official journal of the Academy of Surgical Research. 34(12) |
| ISSN: | 1521-0553 |
| Popis: | Remote ischemic preconditioning (rIPC) is a preventive strategy against ischemia-reperfusion injury. To reduce ischemia-reperfusion injury of random-pattern skin flaps, we investigated the therapeutic effects of rIPC combined with human adipose-derived stem cells (hADSCs) in a rat model.In total, 24 female Sprague Dawley rats were divided into four groups (n = 6 each): control (skin flap only), rIPC, hADSCs, and rIPC + hADSCs. rIPC was performed in the hind limb of the rats over three cycles of 5 min of occlusion and 5 min of reperfusion, using a tourniquet. A rectangular (3 × 9 cm) dorsal skin flap was used. hADSCs (5 × 10The necrotic area of the skin flap significantly decreased in the rIPC + hADSCs group (32.75 ± 1.43%) compared with the control (40.60 ± 3.27%, P 0.01) and rIPC groups (38.84 ± 0.77%, P 0.05). Dye-labeled hADSCs migrated to the skin flap from the injection site. In the rIPC + hADSCs group, the epithelial tissue and skin appendage had regenerated, and the smooth muscle and subcutaneous fat layers were preserved. Many more vWF- and CD31-positive vessels were observed in the rIPC + hADSCs group compared with the other groups.The rIPC + hADSCs treatment appeared to reduce skin flap necrosis and activated neovascularization in rats. Therefore, it may be a good strategy for clinical treatment of ischemia-reperfusion injury. |
| Databáze: | OpenAIRE |
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