Proton Magnetic Resonance Spectroscopy of Polymorphonuclear Leukocytes from Patients with Serious Bacterial Infections
| Autor: | Krystyna Sztelma, Miriam L. Paul, Tania C. Sorrell, George L. May |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Lipopolysaccharides
medicine.medical_specialty Necrosis Lipopolysaccharide Neutrophils medicine.medical_treatment Gastroenterology Proinflammatory cytokine chemistry.chemical_compound In vivo Internal medicine medicine Humans Immunology and Allergy Interleukin 6 biology Interleukin-6 Tumor Necrosis Factor-alpha business.industry Bacterial Infections medicine.disease Magnetic Resonance Imaging Infectious Diseases Cytokine chemistry Bacteremia Immunology biology.protein Tumor necrosis factor alpha medicine.symptom business |
| Zdroj: | Journal of Infectious Diseases. 168:386-392 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/168.2.386 |
| Popis: | Triacylglycerols in human neutrophils exposed to proinflammatory stimuli generate a high-resolution proton magnetic resonance (1H MR) spectrum. Lipid cross-peak F volumes in neutrophils from patients with inflammatory conditions were measured. Values in patients hospitalized with localized infections (14.4 +/- 9.0; mean +/- SD) or bacteremia (19.3 +/- 9.7) were significantly higher than in patients with noninflammatory conditions (6.2 +/- 5.3) and healthy controls (2.0 +/- 3.0; P.001). The positive predictive value of F volumes10 was 93% for all infection; the negative predictive value of volumesor = 10 was 68% for all infection and 92% for bacteremia. Plasma lipopolysaccharide (LPS) concentrations were highest in bacteremic patients but did not correlate with levels of tumor necrosis factor-alpha (TNF alpha) or interleukin-6. In vitro, LPS increased F volumes of control neutrophils from 2.0 +/- 3.0 to 37.2 +/- 6.7 (P.001); TNF alpha had no effect. F volumes in 1H MR spectra may be useful clinically to discriminate between serious bacterial infection and other inflammatory conditions. TNF alpha is not the stimulus for generation of lipid spectra in vivo. |
| Databáze: | OpenAIRE |
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