The acute effects of strength, endurance and concurrent exercises on the Akt/mTOR/p70S6K1 and AMPK signaling pathway responses in rat skeletal muscle

Autor: Valmor Tricoli, Marcelo G. Pereira, C.R. Bueno Junior, Marcelo Saldanha Aoki, Edilamar Menezes de Oliveira, Hamilton Roschel, E. O. de Souza, P.C. Brum, C. Urginowitsch
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Male
AMPK
medicine.medical_specialty
Cell signaling
Physiology
Immunology
Immunoblotting
Biophysics
P70-S6 Kinase 1
Biology
AMP-Activated Protein Kinases
Biochemistry
AMP-activated protein kinase
Internal medicine
Physical Conditioning
Animal
medicine
Animals
Concurrent training
Muscle Strength
General Pharmacology
Toxicology and Pharmaceutics
Phosphorylation
Rats
Wistar
Muscle
Skeletal
Protein kinase B
lcsh:QH301-705.5
PI3K/AKT/mTOR pathway
lcsh:R5-920
Strength exercise
General Neuroscience
TOR Serine-Threonine Kinases
Skeletal muscle
Ribosomal Protein S6 Kinases
70-kDa
Biomedical Sciences
Cell Biology
General Medicine
Rats
Endocrinology
medicine.anatomical_structure
lcsh:Biology (General)
biology.protein
Physical Endurance
Plantaris muscle
lcsh:Medicine (General)
Protein Kinases
Proto-Oncogene Proteins c-akt
Zdroj: Brazilian Journal of Medical and Biological Research, Vol 46, Iss 4, Pp 343-347 (2013)
Scopus-Elsevier
Brazilian Journal of Medical and Biological Research v.46 n.4 2013
Brazilian Journal of Medical and Biological Research
Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
Brazilian Journal of Medical and Biological Research, Volume: 46, Issue: 4, Pages: 343-347, Published: 19 APR 2013
Popis: The activation of competing intracellular pathways has been proposed to explain the reduced training adaptations after concurrent strength and endurance exercises (CE). The present study investigated the acute effects of CE, strength exercises (SE), and endurance exercises (EE) on phosphorylated/total ratios of selected AMPK and Akt/mTOR/p70S6K1 pathway proteins in rats. Six animals per exercise group were killed immediately (0 h) and 2 h after each exercise mode. In addition, 6 animals in a non-exercised condition (NE) were killed on the same day and under the same conditions. The levels of AMPK, phospho-Thr172AMPK (p-AMPK), Akt, phospho-Ser473Akt (p-Akt), p70S6K1, phospho-Thr389-p70S6K1 (p-p70S6K1), mTOR, phospho-Ser2448mTOR (p-mTOR), and phospho-Thr1462-TSC2 (p-TSC2) expression were evaluated by immunoblotting in total plantaris muscle extracts. The only significant difference detected was an increase (i.e., 87%) in Akt phosphorylated/total ratio in the CE group 2 h after exercise compared to the NE group (P = 0.002). There were no changes in AMPK, TSC2, mTOR, or p70S6K1 ratios when the exercise modes were compared to the NE condition (P ≥ 0.05). In conclusion, our data suggest that low-intensity and low-volume CE might not blunt the training-induced adaptations, since it did not activate competing intracellular pathways in an acute bout of strength and endurance exercises in rat skeletal muscle.
Databáze: OpenAIRE
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