The Major Cold Shock Gene, cspA , Is Involved in the Susceptibility of Staphylococcus aureus to an Antimicrobial Peptide of Human Cathepsin G
| Autor: | William M. Shafer, Jacqueline T. Balthazar, Samera Bowers, Samuel Katzif, Damien Danavall |
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| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Staphylococcus aureus Cathepsin G Transcription Genetic Molecular Sequence Data Immunology Mutant Sigma Factor Bacillus subtilis In Vitro Techniques medicine.disease_cause Microbiology chemistry.chemical_compound Bacterial Proteins Drug Resistance Bacterial medicine Humans Amino Acid Sequence Gene Escherichia coli Southern blot Base Sequence Sequence Homology Amino Acid biology Serine Endopeptidases Proteins Cold-shock domain biology.organism_classification Cathepsins Molecular Pathogenesis Peptide Fragments Anti-Bacterial Agents Cold Temperature Mutagenesis Insertional Infectious Diseases chemistry Genes Bacterial Intercellular Signaling Peptides and Proteins Parasitology |
| Zdroj: | Infection and Immunity. 71:4304-4312 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | A Tn 551 insertional library of Staphylococcus aureus strain ISP479 was challenged with an antimicrobial peptide (CG 117-136) derived from human neutrophil cathepsin G (CG). After repeated selection and screening of surviving colonies, a mutant was identified that expressed increased resistance to CG 117-136. Southern hybridization analysis revealed that the Tn 551 insert in this mutant (SK1) was carried on a 10.6-kb Eco RI chromosomal DNA fragment. Subsequent physical mapping of this Tn 551 insert revealed that it was positioned between a putative promoter sequence and the translational start codon of the cspA gene, which encodes a protein (CspA) highly similar to the major cold shock proteins CspA and CspB of Escherichia coli and Bacillus subtilis , respectively. This Tn 551 insertion as well as a separate deletion-insertion mutation in cspA decreased the capacity of S. aureus to respond to the stress of cold shock and increased resistance to CG 117-136. The results indicate for the first time that a physiologic link exists between bacterial susceptibility to an antimicrobial peptide and a stress response system. |
| Databáze: | OpenAIRE |
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