Analysis of Ipl1-Mediated Phosphorylation of the Ndc80 Kinetochore Protein in Saccharomyces cerevisiae
| Autor: | Jeffrey A. Ranish, Sue Biggins, Bungo Akiyoshi, Christian R. Nelson |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
inorganic chemicals
Saccharomyces cerevisiae Proteins Aurora B kinase Saccharomyces cerevisiae macromolecular substances Protein Serine-Threonine Kinases Biology Microtubules environment and public health Aurora Kinases Microtubule Notes Genetics Protein phosphorylation Amino Acid Sequence Phosphorylation Nuclear protein Kinetochores Kinetochore Intracellular Signaling Peptides and Proteins Nuclear Proteins NDC80 enzymes and coenzymes (carbohydrates) Spindle checkpoint Mutation bacteria |
| Zdroj: | Genetics. 183:1591-1595 |
| ISSN: | 1943-2631 |
| DOI: | 10.1534/genetics.109.109041 |
| Popis: | Phosphorylation of the Ndc80 kinetochore protein by the Ipl1/Aurora B kinase reduces its microtubule binding activity in vitro. We found that kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo, but this phosphorylation is not essential. Instead, we show that additional Ipl1 targets contribute to segregation and the spindle checkpoint. |
| Databáze: | OpenAIRE |
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