Discovery of thymosin β4 as a human exerkine and growth factor
| Autor: | Marie Björnholm, Jonas T. Treebak, Atul S. Deshmukh, Niklas Rye Jørgensen, Christoffer Clemmensen, Anna Krook, Alba Gonzalez-Franquesa, Juleen R. Zierath, Emilie Dalbram, Håkan Karlsson, Stanislava Pankratova, Ben Stocks, Morten Hostrup, Sevda Gheibi, Alexander V. Chibalin, Ankita Agrawal, Thomas Nielsen, Melissa L. Borg, Michael S. Kuefner |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Proteomics Physiology medicine.medical_treatment Muscle Fibers Skeletal Neuronal Outgrowth Muscular Diseases Tandem Mass Spectrometry Cell Line Tumor Faculty of Science medicine Animals Humans Endocrine system Secreted factor Muscle Skeletal Exercise Cell Proliferation Osteoblasts Chemistry Growth factor Skeletal muscle Cell Biology Cell biology Mice Inbred C57BL Thymosin Thymosin beta-4 Disease Models Animal medicine.anatomical_structure Diabetes Mellitus Type 2 Muscle contraction Case-Control Studies Physical Endurance Insulin Resistance medicine.symptom Exerkine Muscle Contraction Signal Transduction |
| Zdroj: | Gonzalez-Franquesa, A, Stocks, B, Borg, M L, Kuefner, M, Dalbram, E, Nielsen, T S, Agrawal, A, Pankratova, S, Chibalin, A V, Karlsson, H K R, Gheibi, S, Björnholm, M, Jørgensen, N R, Clemmensen, C, Treebak, J T, Hostrup, M, Krook, A, Zierath, J R & Deshmukh, A S 2021, ' Discovery of thymosin β4 as a human exerkine and growth factor ', American Journal of Physiology: Cell Physiology, vol. 321, no. 5, pp. C770-C778 . https://doi.org/10.1152/ajpcell.00263.2021 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.00263.2021 |
| Popis: | Skeletal muscle is an endocrine organ secreting exercise-induced factors (exerkines), which play a pivotal role in interorgan cross talk. Using mass spectrometry (MS)-based proteomics, we characterized the secretome and identified thymosin β4 (TMSB4X) as the most upregulated secreted protein in the media of contracting C2C12 myotubes. TMSB4X was also acutely increased in the plasma of exercising humans irrespective of the insulin resistance condition or exercise mode. Treatment of mice with TMSB4X did not ameliorate the metabolic disruptions associated with diet induced-obesity, nor did it enhance muscle regeneration in vivo. However, TMSB4X increased osteoblast proliferation and neurite outgrowth, consistent with its WADA classification as a prohibited growth factor. Therefore, we report TMSB4X as a human exerkine with a potential role in cellular cross talk. |
| Databáze: | OpenAIRE |
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