Preparation and Characterization of Polyamidoamine G2.0-Hematin as a Biocatalyst for Fabricating Catecholic Gelatin Hydrogel

Autor:	Van Toan Nguyen, Bao Huy Le, Tan Phuoc Ton, Phuong Doan, Thi Yen Nhi Tran, Dinh Trung Nguyen, Ngoc Quyen Tran, Bich Tram Nguyen, Kim Thi Hoang Nguyen, Le Hang Dang
Rok vydání:	2021
Předmět:	food.ingredient Article Subject Polymers and Plastics biology Chemistry Chemical technology Chemical structure TP1-1185 Adhesion Gelatin Horseradish peroxidase chemistry.chemical_compound food Pyrogallol Dendrimer Self-healing hydrogels biology.protein Zeta potential Nuclear chemistry
Zdroj:	International Journal of Polymer Science, Vol 2021 (2021)
ISSN:	1687-9430 1687-9422
Popis:	In this study, we report that an enzyme-mimicking biocatalyst polyamidoamine (PAMAM) dendrimer G2.0-hematin (G2.0-He) was fabricated successfully. The chemical structure of G2.0-He was verified by 1H NMR and FT-IR spectroscopy. G2.0-He exhibited a size distribution from 11.6 ± 1.7 nm to 12.5 ± 2.9 nm and a zeta potential from 32.5 mV to 25.6 mV along with the enhancement of the hematin conjugation degree. The relative activity of G2.0-He was evaluated based on pyrogallol oxidation reactions at pH = 7 . The results showed that G2.0-He was more stable than horseradish peroxidase (HRP) enzyme in high H2O2 concentrations. The HRP-mimic ability of G2.0-He was also confirmed by the catalyzation when preparing catecholic gelatin hydrogels under mild conditions. Moreover, our results also revealed that these hydrogels performed with excellent cytocompatibility in an in vitro study and could be used as a potential scaffold for adhesion and proliferation of fibroblast cells. The obtained results indicated that G2.0-He is a suitable platform for altering the HRP enzyme in several biomedical applications.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e31f4d7f1e4ac713a614824fea12a218 https://doi.org/10.1155/2021/5563229 Zobrazit plný text záznamu Plný text