Baculovirus entire ORF1629 is not essential for viral replication

Autor: Jae Bang Choi, Yeon Ho Je, Sung Min Bae, Hyun-Soo Kim, Beom Ku Han, Won Seok Gwak, See Nae Lee, Soo Dong Woo
Rok vydání: 2019
Předmět:
viruses
Artificial Gene Amplification and Extension
Virus Replication
Recombinant virus
Polymerase Chain Reaction
Biochemistry
law.invention
chemistry.chemical_compound
law
Homologous Recombination
Polymerase chain reaction
Inclusion Bodies
Recombination
Genetic
Multidisciplinary
biology
Eukaryota
Recombinant Proteins
Nucleic acids
Insects
Recombinant DNA
Medicine
Baculoviridae
Research Article
Arthropoda
DNA recombination
Science
Research and Analysis Methods
Microbiology
Virus
Viral Proteins
Virology
Genetics
Animals
Molecular Biology Techniques
Molecular Biology
fungi
Organisms
Biology and Life Sciences
Proteins
Reproducibility of Results
DNA
Reverse Transcriptase-Polymerase Chain Reaction
biology.organism_classification
Invertebrates
Viral Replication
Autographa californica
Viral replication
chemistry
Homologous recombination
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 8, p e0221594 (2019)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0221594
Popis: It is generally accepted that ORF1629 is essential for baculovirus replication, which has enabled isolation of recombinant viruses in a baculovirus expression system using linearized viral DNA. ORF1629-defective viruses cannot replicate in insect cells; only recombinant virus with complete ORF1629 restoration by recombination can propagate, allowing for pure isolation and the development of bacmids for easy selection of recombinant viruses. We inadvertently found proliferation in insect cells of a bacmid lacking a complete ORF1629. PCR indicated no other viruses but a lack of complete ORF1629 in the proliferated bacmid, suggesting that the baculovirus propagated without a complete ORF1629. Lack of ORF1629 decreased the virus growth rate and yield; it also increased the occlusion body (OB) size but decreased its yield. These results were confirmed for Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Bombyx mori NPV (BmNPV). Thus, entire ORF1629 is not essential for viral replication, though it does affect the virus growth rate, yield, and size and OB production.
Databáze: OpenAIRE
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