Arabidopsis galactinol synthase AtGolS2 improves drought tolerance in the monocot model Brachypodium distachyon
| Autor: | Fumie Mori, Ryo Akashi, Yasuyo Himuro, Takahiro Gondo, Fuminori Takahashi, Kanako Ishiyama, Masatomo Kobayashi, Kazuo Shinozaki |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Plant Somatic Embryogenesis Techniques
Physiology Drought tolerance Arabidopsis Gene Expression Plant Science Genetically modified crops Transformation Genetic Stress Physiological Botany Arabidopsis thaliana biology Arabidopsis Proteins fungi food and beverages Galactosyltransferases Plants Genetically Modified biology.organism_classification Pooideae Droughts Transformation (genetics) Seeds Brachypodium Brachypodium distachyon Agronomy and Crop Science |
| Zdroj: | Journal of Plant Physiology. 171:1127-1131 |
| ISSN: | 0176-1617 |
| DOI: | 10.1016/j.jplph.2014.04.007 |
| Popis: | Brachypodium distachyon (purple false brome) is a herbaceous species belonging to the grass subfamily Pooideae, which also includes major crops like wheat, barley, oat and rye. The species has been established as experimental model organism for understanding and improving cereal crops and temperate grasses. The complete genome of Bd21, the community standard line of B. distachyon, has been sequenced and protocols for Agrobacterium-mediated transformation have been published. Further improvements to the experimental platform including better evaluation systems for transgenic plants are still needed. Here we describe the growth conditions for Bd21 plants yielding highly responsive immature embryos that can generate embryogenic calli for transformation. A prolonged 20-h photoperiod produced seeds with superior immature embryos. In addition, osmotic treatment of embryogenic calli enhanced the efficiency of transfection by particle bombardment. We generated transgenic plants expressing Arabidopsis thaliana galactinol synthase 2 (AtGolS2) in these experiments. AtGolS2-expressing transgenics displayed significantly improved drought tolerance, increasing with increased expression of AtGolS2. These results demonstrate that AtGolS2 can confer drought tolerance to monocots and confirm that Brachypodium is a useful model to further explore ways to understand and improve major monocot crop species. |
| Databáze: | OpenAIRE |
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