Comparison of Various Chromatographic Systems for Analysis of Cytisine in Human Serum, Saliva and Pharmaceutical Formulation by HPLC with Diode Array, Fluorescence or Mass Spectrometry Detection

Autor: Tomasz Tuzimski, Karol Wróblewski, Grzegorz Buszewicz, Dominika Przygodzka, Anna Petruczynik, Piotr Tutka, Patrycjusz Kołodziejczyk
Rok vydání: 2019
Předmět:
Serum
cytisine
Ion chromatography
Pharmaceutical Science
pharmaceutical preparation
Pharmaceutical formulation
HPLC-DAD
01 natural sciences
High-performance liquid chromatography
Fluorescence
Mass Spectrometry
Article
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
Cytisine
chemistry.chemical_compound
Alkaloids
0302 clinical medicine
lcsh:Organic chemistry
Drug Discovery
HPLC-FLD
Humans
Protein precipitation
030212 general & internal medicine
Solid phase extraction
Physical and Theoretical Chemistry
Chromatography
High Pressure Liquid
saliva
Chromatography
HPLC-MS/MS
Chemistry
Elution
Hydrophilic interaction chromatography
010401 analytical chemistry
Organic Chemistry
Chromatography
Ion Exchange
Azocines
0104 chemical sciences
Pharmaceutical Preparations
optimisation of chromatographic systems
Chemistry (miscellaneous)
retention mechanism
Molecular Medicine
SPE
Hydrophobic and Hydrophilic Interactions
Quinolizines
Zdroj: Molecules
Molecules, Vol 24, Iss 14, p 2580 (2019)
Volume 24
Issue 14
ISSN: 1420-3049
DOI: 10.3390/molecules24142580
Popis: Background: Identification and quantitative determination of cytisine, especially in biological samples and pharmaceutical formulations, is still a difficult analytical task. Cytisine is an alkaloid with a small and very polar molecule. For this reason, it is very weakly retained on reversed phase (RP) stationary phases, such as commonly used alkyl-bonded phases. The very weak retention of cytisine causes it to be eluted together with the components of biological matrices. Objective: Comparison and evaluation of various chromatographic systems for analysis of cytisine in different matrices&mdash
serum, saliva and pharmaceutical formulation&mdash
by high performance liquid chromatography (HPLC) with diode array (DAD), fluorescence (FLD) and mass spectrometry (MS) detection. Methods: The analyses were performed using HPLC in reversed phase (RP), hydrophilic interaction liquid chromatography (HILIC) and ion exchange chromatography (IEC) modes. Different sample pre-treatment methods were tested: Protein precipitation (with acetone, methanol (MeOH) or acetonitrile (ACN), and solid phase extraction (SPE) using cartridges with octadecyl (C18), hydrophilic-lipophilic balanced copolymer (HLB) or strong cation exchange sorbents (Strata X-C). Conclusion: Significant differences were observed in retention parameters with a change of the used chromatographic system. The various properties of stationary phases resulted in differences in analyte retention, peaks&rsquo
shape and systems&rsquo
efficiency. The weakest retention was observed using RP systems
however, the use of the Polar RP phase can be an alternative for application in green chromatography. In the strongest retention was observed using a strong cation exchange (SCX) phase. The most optimal systems were chosen for the analysis of cytisine in the pharmaceutical preparation, serum and saliva after sample pre-treatment with the new SPE procedure. Due to the sensitivity, the use of HPLC-DAD or HPLC-FLD is the most optimal for drug analysis in pharmaceutical preparations, whereas HPLC-MS is suitable for analysis of cytisine in biological samples.
Databáze: OpenAIRE
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