Glycoprotein IIb/IIIa and P2Y12Induction by Oligochitosan Accelerates Platelet Aggregation
| Autor: | Nik Soriani Yaacob, Ahmad Hazri Abdul Rashid, Arman Zaharil Mat Saad, Zanariah Ujang, Ahmad Sukari Halim, Mercy Halleluyah Periayah, Abdul Rahim Hussein |
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| Rok vydání: | 2014 |
| Předmět: |
Blood Platelets
Article Subject Platelet Aggregation Oligosaccharides lcsh:Medicine Chitin Platelet Glycoprotein GPIIb-IIIa Complex Biology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Adenosine Triphosphate P2Y12 Cell surface receptor Humans Platelet Cells Cultured Chitosan General Immunology and Microbiology lcsh:R General Medicine Molecular biology Receptors Purinergic P2Y12 Adenosine diphosphate Coagulation chemistry Hemostasis Glycoprotein IIb/IIIa Research Article |
| Zdroj: | BioMed Research International, Vol 2014 (2014) BioMed Research International |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Platelet membrane receptor glycoprotein IIb/IIIa (gpiibiiia) is a receptor detected on platelets. Adenosine diphosphate (ADP) activates gpiibiiia and P2Y12, causing platelet aggregation and thrombus stabilization during blood loss. Chitosan biomaterials were found to promote surface induced hemostasis and were capable of activating blood coagulation cascades by enhancing platelet aggregation. Our current findings show that the activation of the gpiibiiia complex and the major ADP receptor P2Y12is required for platelet aggregation to reach hemostasis following the adherence of various concentrations of chitosan biomaterials [7% N,O-carboxymethylchitosan (NO-CMC) with 0.45 mL collagen, 8% NO-CMC, oligochitosan (O-C), and oligochitosan 53 (O-C 53)]. We studied gpiibiiia and P2Y12through flow cytometric analysis and western blotting techniques. The highest expression of gpiibiiia was observed with Lyostypt (74.3 ± 7.82%), followed by O-C (65.5 ± 7.17%). Lyostypt and O-C resulted in gpiibiiia expression increases of 29.2% and 13.9%, respectively, compared with blood alone. Western blot analysis revealed that only O-C 53 upregulated the expression of P2Y12(1.12 ± 0.03-fold) compared with blood alone. Our findings suggest that the regulation of gpiibiiia and P2Y12levels could be clinically useful to activate platelets to reach hemostasis. Further, we show that the novel oligochitosan is able to induce the increased expression of gpiibiiia and P2Y12, thus accelerating platelet aggregationin vitro. |
| Databáze: | OpenAIRE |
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