Validated liquid chromatographic method for the determination of bexarotene in human plasma
| Autor: | G. Wilkens, N. C. Van De Merbel, G. Loewen, J. H. Van Veen |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Analyte
Tetrahydronaphthalenes Clinical Biochemistry Analytical chemistry Mass spectrometry Sensitivity and Specificity Biochemistry Analytical Chemistry chemistry.chemical_compound Humans Sample preparation Acetonitrile Chromatography Chemistry Extraction (chemistry) Reproducibility of Results Cell Biology General Medicine Reference Standards Isoamyl alcohol Pentane Hexane Spectrometry Fluorescence Bexarotene Calibration Chromatography Liquid |
| Zdroj: | Journal of Chromatography B. 775:189-195 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/s1570-0232(02)00291-x |
| Popis: | A new liquid chromatographic method is described for the determination of the anti-tumour agent bexarotene in human plasma over the range 0.500–1500 ng/ml, using 1 ml of sample. Sample preparation consists of liberating the analyte from plasma lipids by adding acetonitrile, followed by acidification of the plasma and liquid extraction using a mixture of isoamyl alcohol and pentane or hexane. Separation and quantitation are performed by reversed-phase column liquid chromatography with fluorescence detection. Parameters affecting the performance of these steps are discussed. Validation results on linearity, selectivity, accuracy, precision, recovery and stability are shown, as well as the application of the method to samples from clinical trials. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect