Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection
| Autor: | Michiko Hirose, Kimiko Inoue, Akie Shinmen, Atsuo Ogura, Keiji Mochida, Masao Ito, Hiromi Miki, Kuniya Abe, Narumi Ogonuki, Arata Honda, Mika Ohkawa, Misako Yuzuriha |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Genotype Offspring medicine.medical_treatment Embryonic Development Embryonic Structures Biology DNA Mitochondrial Intracytoplasmic sperm injection Cryopreservation Cell Line Andrology Mice Genetics medicine Animals Sperm Injections Intracytoplasmic reproductive and urinary physiology Embryonic Stem Cells Polymorphism Genetic Chimera Embryo Cell Biology Embryonic stem cell Sperm Spermatozoa Mice Mutant Strains Phenotype Cell culture Female Developmental biology Developmental Biology Semen Preservation |
| Zdroj: | Molecular reproduction and development. 74(9) |
| ISSN: | 1040-452X |
| Popis: | Recently, mice and embryonic stem (ES) cells with allelic polymorphisms have been used extensively in the field of genetics and developmental biology. In this study, we examined whether intersubspecific hybrid mice and ES cells with these genotypes can be efficiently produced by intracytoplasmic sperm injection (ICSI). Frozen-thawed spermatozoa from wild-derived strains, JF1 (Mus musculus molossinus), MSM (M. m. molossinus), HMI (M. m. castaneus), and SWN (M. m. spp.), were directly injected into mature oocytes from laboratory mice ((C57BL/6 � DBA2)F1; M. m. domesticus). The in vitro and in vivo developmental capacity of F1 embryos was not significantly different among the groups (P >0.05), and term offspring were efficiently obtained in all groups (27%-34% of transferred embryos). However, the mean body and placental weights of the offspring differed significantly with genotype (P |
| Databáze: | OpenAIRE |
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