Homologous acellular matrix graft for vaginal repair in rats: a pilot study for a new reconstructive approach
| Autor: | Rajvir Dahiya, Joerg Wefer, N. Schlote, Emil A. Tanagho, Karl-Dietrich Sievert, Lora Nunes, Noritoshi Sekido, Udo Jonas |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Acellular matrix Urology medicine.medical_treatment Urinary Bladder Vaginal Diseases Lumen (anatomy) Matrix (biology) Rats Sprague-Dawley Homologous chromosome Medicine Animals Wound Healing Urinary bladder Hysterectomy business.industry Plastic Surgery Procedures Vaginal repair Surgery Extracellular Matrix Rats Disease Models Animal medicine.anatomical_structure Vagina Feasibility Studies Female business |
| Zdroj: | World journal of urology. 20(4) |
| ISSN: | 0724-4983 |
| Popis: | The purpose of this paper is to investigate using an acellular matrix graft of vagina (VAMG) or bladder (BAMG) in vaginal reconstruction. In 18 rats, vaginal length was measured and a hysterectomy performed. In three control animals, the vaginal stump was closed. In eight rats, the vagina was augmented with a VAMG; in seven, a BAMG was used. After 2-12 weeks, the animals were sacrificed, the vaginal length was reevaluated, and the vaginas were prepared for histologic evaluation. In the controls, the vagina was markedly shorter postoperatively. In the grafted animals, vaginal length was not significantly less than preoperative values with either matrix. Epithelialization, vascularization, and alpha-actin expression in the grafts were consistently observed. Regeneration appeared to be slightly greater in the organ-specific vagina matrix. With either matrix, however, although the vaginal stump remained open, the grafts lost most of the lumen. Vaginal reconstruction with a vagina acellular matrix graft is technically feasible. If further experiments can address the problem of luminal collapse - with, for instance, tissue expanders in the matrix - this technique may offer an alternative to the complex therapeutic options currently available. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink