Guidelines for best practice in placebo-controlled experimental studies on probiotics in rodent animal models
Autor: | E M Myles, Thomas A. Tompkins, Amanda Piano, V. de Carvalho, I D Romkey, Tara S. Perrot, M E O'Leary |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Microbiology (medical) Animal Experimentation Diarrhea medicine.medical_specialty business.industry Best practice Probiotics Rodentia Research findings Placebo Microbiology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Behavioural testing Laboratory Animal Science Models Animal Medicine Animals 030211 gastroenterology & hepatology Animal studies business Intensive care medicine Interpretability |
Zdroj: | Beneficial microbes. 11(3) |
ISSN: | 1876-2891 |
Popis: | In the absence of established best practice standards in the probiotic field for reducing the risk of bacterial transfer between experimental groups, we developed protocols and methods to ensure the highest quality and interpretability of results from animal studies, even when performed in non-conventional animal care facilities. We describe easily implementable methods for reducing cross-contamination during animal housing, behavioural testing, and euthanasia, along with highlighting protocols for contamination detection in experimental subjects and laboratory areas using qPCR. In light of the high cross-contamination risks between animals during experiments involving probiotics, constant vigilance in animal care and research protocols is critical to ensure valid and reliable research findings. |
Databáze: | OpenAIRE |
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