Polychlorinated biphenyls alter the expression of endothelial nitric oxide synthase mRNA in human umbilical vein endothelial cells
| Autor: | Hideki Fukata, Keiko Aida-Yasuoka, Naoko Omori, Koji Yamazaki, Motone Kuriyama, Chisato Mori, Koji Sato, Hidetaka Takigami, Masaharu Ichinose |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Umbilical Veins Nitric Oxide Synthase Type III Health Toxicology and Mutagenesis Estrogen receptor Toxicology Umbilical vein 03 medical and health sciences 0302 clinical medicine Enos Internal medicine medicine Cytochrome P-450 CYP1A1 Humans Receptors Cholinergic RNA Messenger Receptor Fulvestrant Cells Cultured Benzoflavones Fetus 030102 biochemistry & molecular biology biology Estradiol Endothelial Cells General Medicine biology.organism_classification Aryl hydrocarbon receptor Polychlorinated Biphenyls Nitric oxide synthase Endothelial stem cell Endocrinology 030220 oncology & carcinogenesis biology.protein |
| Zdroj: | Humanexperimental toxicology. 26(10) |
| ISSN: | 0960-3271 |
| Popis: | Polychlorinated biphenyls (PCBs) are a group of persistent pollutants that are detected in maternal serum and umbilical cord, suggesting that fetal exposure also needs to be considered. The effects of dioxin-like PCB congeners 3,3',4,4'-tetrachlorobiphenyl (PCB77) and 3,3',4,4',5-pentachlorobiphenyl (PCB126) and a non-dioxin-like compound 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153) on the expression of endothelial nitric oxide synthase (eNOS), known to maintain blood flow to the fetus, in human umbilical vein endothelial cells (HUVECs) were investigated. The mRNA levels of eNOS, aryl hydrocarbon receptor (AhR) and cytochrome P450 (CYP) 1A1 in cells treated with 5 µM PCBs for 24 hours were analysed by real-time RT-PCR. Cells were also treated with α-naphthoflavone (α NF), an AhR antagonist or ICI 182780, an estrogen receptor (ER) antagonist, one hour prior to PCB exposure, to observe the effects of these receptors on eNOS modulation. Each PCB increased the eNOS mRNA level by 4.5-fold that was markedly inhibited by αNF. ERs were also suspected of altering eNOS levels because ICI 182780 treatment resulted in a decrease in the eNOS level. These results suggest that the eNOS mRNA expression increases due to the action of PCBs related to both AhR and ERs in HUVECs, and that maternal PCB exposure could influence fetal circulation. Human & Experimental Toxicology (2007) 26, 811— 816 |
| Databáze: | OpenAIRE |
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