Methotrexate reduces keratinocyte proliferation, migration and induces apoptosis in HaCaT keratinocytes in vitro and reduces wound closure in Skh1 mice in vivo
| Autor: | Martin Zapata-Zúñiga, Diana P. Portales-Pérez, Salvador García-Cruz, Fátima Ochoa-González, Julio Enrique Castañeda-Delgado, Julio C. Fernández-Ruiz, José Antonio Enciso-Moreno, Lorena Troncoso-Vázquez, Alberto R. Cervantes-Villagrana, Mitzzy F. Medellín-Luna, Carmen J. Serrano |
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| Rok vydání: | 2021 |
| Předmět: |
Keratinocytes
Apoptosis Dermatology Statistics Nonparametric Pathology and Forensic Medicine Mice 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine In vivo medicine Animals Clonogenic assay Cell Proliferation Analysis of Variance Wound Healing 030504 nursing Cell growth business.industry Cell cycle Disease Models Animal HaCaT Methotrexate medicine.anatomical_structure Cancer research 0305 other medical science Keratinocyte Wound healing business |
| Zdroj: | Journal of Tissue Viability. 30:51-58 |
| ISSN: | 0965-206X |
| Popis: | The aim of the present work was to evaluate MTX treatment (0.1, 1 and 10 μg mL−1) in vitro in order to characterize its effects on cell proliferation alterations in cell cycle of HaCaT keratinocytes and wound healing in a Skh1 mice treated with MTX (low doses 30 mg kg−1, high doses 200 mg kg−1 and repeated doses at 1.5 mg kg−1). We analyzed the cytotoxic effect of methotrexate by a resazurin assay. The effects in the proliferation, cell cycle and apoptosis of HaCaT cells were analyzed by flow cytometry. The effects of MTX on wound healing in vivo were also analyzed. A trend toward reduction in the resazurin assay was found (p > 0.05). Reduced proliferation was also identified in a clonogenic assay and a CFSE assay (p |
| Databáze: | OpenAIRE |
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