Two showy traits, scent emission and pigmentation, are finely coregulated by the MYB transcription factor PH 4 in petunia flowers
| Autor: | Alon Cna'ani, Marianna Ovadis, Ben Spitzer-Rimon, Jasmin Ravid, Moran Farhi, Alexander Vainstein, Javiera Aravena-Calvo, Tania Masci |
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| Rok vydání: | 2015 |
| Předmět: |
Volatile Organic Compounds
Phenylpropanoid Pigmentation Physiology fungi food and beverages Context (language use) Flowers Plant Science Biology biology.organism_classification Petunia Vacuolar acidification Botany Gene silencing MYB Gene Silencing Gene Transcription factor Plant Proteins Transcription Factors |
| Zdroj: | New Phytologist. 208:708-714 |
| ISSN: | 1469-8137 0028-646X |
| DOI: | 10.1111/nph.13534 |
| Popis: | The mechanism underlying the emission of phenylpropanoid volatiles is poorly understood. Here, we reveal the involvement of PH4, a petunia MYB-R2R3 transcription factor previously studied for its role in vacuolar acidification, in floral volatile emission. We used the virus-induced gene silencing (VIGS) approach to knock down PH4 expression in petunia, measured volatile emission and internal pool sizes by GC-MS, and analyzed transcript abundances of scent-related phenylpropanoid genes in flowers. Silencing of PH4 resulted in a marked decrease in floral phenylpropanoid volatile emission, with a concurrent increase in internal pool levels. Expression of scent-related phenylpropanoid genes was not affected. To identify putative scent-related targets of PH4, we silenced PH5, a tonoplast-localized H(+) -ATPase that maintains vacuolar pH homeostasis. Suppression of PH5 did not yield the reduced-emission phenotype, suggesting that PH4 does not operate in the context of floral scent through regulation of vacuolar pH. We conclude that PH4 is a key floral regulator that integrates volatile production and emission processes and interconnects two essential floral traits - color and scent. |
| Databáze: | OpenAIRE |
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