Peroxidation of Synaptosomes Alters the Dopamine Uptake Complex but Spares the Exocytotic Release of Dopamine
| Autor: | Jean Costentin, Franck Girbe, Charles Ramassamy, Yves Christen |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Dopamine Potassium chemistry.chemical_element Pharmacology Exocytosis Piperazines Pathology and Forensic Medicine Mice Radioligand Assay GBR-12783 chemistry.chemical_compound Dopamine Uptake Inhibitors Dopamine Uptake Complex medicine Animals Incubation Analysis of Variance Chemistry Ascorbic acid Free radical scavenger Neuropsychology and Physiological Psychology Biochemistry Calcium Lipid Peroxidation Neurology (clinical) Trolox Synaptosomes medicine.drug |
| Zdroj: | Neurodegeneration. 4:155-160 |
| ISSN: | 1055-8330 |
| DOI: | 10.1006/neur.1995.0019 |
| Popis: | Synaptosomes, prepared from the striata of mice, and incubated for 1 h in a Krebs-Ringer medium with the peroxidative combination of ascorbic acid (0.1 mM)/Fe2+ (1 microM), lose their ability to take up [3H] dopamine. This effect is associated with a decrease in binding of the dopamine uptake inhibitor [3H] GBR 12783. The free radical scavenger trolox C (0.1 mM) and the Ginkgo biloba extract EGb 761 (10 micrograms/ml) prevent both effects. Although submitted to these peroxidative conditions after loading with [3H] DA, superfused synaptosomes retain their ability to release [3H] DA when depolarized by high potassium concentrations (40 mM). This release is higher than that observed when synaptosomes are incubated without ascorbic acid/Fe2+, and does not seem to depend upon peroxidation, since it is also observed when incubation is performed in the presence of the free radical scavengers EGb 761 (10 micrograms/ml) and trolox C (0.1 mM). |
| Databáze: | OpenAIRE |
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