Effect of permethrin insecticide on rat polymorphonuclear neutrophils
| Autor: | Cinzia Nasuti, Isuke Imada, Maria Letizia Falcioni, Franco Cantalamessa, Masayasu Inoue, Rosita Gabbianelli |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Insecticides Antioxidant Membrane Fluidity Neutrophils Phagocytosis medicine.medical_treatment Apoptosis Inflammation Biology Pharmacology Toxicology Cell Degranulation chemistry.chemical_compound Superoxides medicine Animals Rats Wistar Permethrin chemistry.chemical_classification Reactive oxygen species NADPH oxidase Superoxide Hydrogen Peroxide General Medicine Rats Respiratory burst Biochemistry chemistry biology.protein medicine.symptom Oxidation-Reduction medicine.drug |
| Zdroj: | Chemico-Biological Interactions. 182:245-252 |
| ISSN: | 0009-2797 |
| Popis: | Polymorphonuclear neutrophils are professional phagocytes whose efficacy depends on a multicomponent NADPH oxidase for generating superoxide anions and bacterial killing. They can be primed and activated by different agents that can impair oxidative burst and phagocytosis with opposite effects: reduced capability to destroy bacteria or hyperactivation that induces the generation of large quantities of toxic reactive oxygen species, which can damage surrounding tissue and participate in inflammation. The present study was designed to evaluate the effect of sub-chronic (60 days) permethrin treatment (1/10 DL(50)) on rat polymorphonuclear neutrophils respiratory burst. The results show that permethrin treatment increases superoxide anion production (33 times) and the activity of hydrogen peroxide-myeloperoxidase system (67 times). In vitro experiments suggest that this effect can be related to permethrin priming and to physico-chemical changes at the plasma membrane level of neutrophils. The antioxidant supplementation with Vitamin E and coenzyme Q(10) can protect against the abnormal respiratory burst in rat treated with permethrin. The in vitro studies show that neutrophil apoptosis begins soon after 1h of incubation with permethrin (0.725% of total cells) or its metabolites (3-phenoxybenzyl alcohol, 3-phenoxybenzaldehyde and 3-phenoxybenzoic acid 1.36, 2.26 and 1.3 of total cells, respectively) and that the level of apoptotic cells is very low. In conclusion, immunotoxicity of permethrin measured in rats could prompt future studies on the consequences of chronic insecticide exposure. |
| Databáze: | OpenAIRE |
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