Correction: Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation

Autor: Angélica M. Jaramillo, Santiago Sierra, Paul Chavarriaga-Aguirre, Diana Katherine Castillo, Anestis Gkanogiannis, Luis Augusto Becerra López-Lavalle, Juan Pablo Arciniegas, Tianhu Sun, Li Li, Ralf Welsch, Erick Boy, Daniel Álvarez
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Pigments
Manihot
Single Nucleotide Polymorphisms
Organic chemistry
Biochemistry
Plant Roots
Database and Informatics Methods
Gene Expression Regulation
Plant
Vegetables
Vitamin A
Materials
Flowering Plants
Plant Proteins
Multidisciplinary
Provitamins
Eukaryota
food and beverages
Vitamins
Plants
Physical sciences
Chemistry
Experimental Organism Systems
Geranylgeranyl-Diphosphate Geranylgeranyltransferase
Medicine
Sequence Analysis
Research Article
Bioinformatics
Arabidopsis Thaliana
Science
Materials Science
Brassica
macromolecular substances
Research and Analysis Methods
Biosynthesis
Genes
Plant
Chemical compounds
Model Organisms
Plant and Algal Models
Organic compounds
Genetics
Cassava
Organic Pigments
organic chemicals
Organisms
Biology and Life Sciences
Correction
Carotenoids
Biosynthetic Pathways
Animal Studies
Sweet Potato
Shrubs
Sequence Alignment
Zdroj: PLoS ONE
PLoS ONE, Vol 17, Iss 1, p e0262412 (2022)
ISSN: 1932-6203
Popis: Cassava (Manihot esculenta Crantz) biofortification with provitamin A carotenoids is an ongoing process that aims to alleviate vitamin A deficiency. The moderate content of provitamin A carotenoids achieved so far limits the contribution to providing adequate dietary vitamin A levels. Strategies to increase carotenoid content focused on genes from the carotenoids biosynthesis pathway. In recent years, special emphasis was given to ORANGE protein (OR), which promotes the accumulation of carotenoids and their stability in several plants. The aim of this work was to identify, characterize and investigate the role of OR in the biosynthesis and stabilization of carotenoids in cassava and its relationship with phytoene synthase (PSY), the rate-limiting enzyme of the carotenoids biosynthesis pathway. Gene and protein characterization of OR, expression levels, protein amounts and carotenoids levels were evaluated in roots of one white (60444) and two yellow cassava cultivars (GM5309-57 and GM3736-37). Four OR variants were found in yellow cassava roots. Although comparable expression was found for three variants, significantly higher OR protein amounts were observed in the yellow varieties. In contrast, cassava PSY1 expression was significantly higher in the yellow cultivars, but PSY protein amount did not vary. Furthermore, we evaluated whether expression of one of the variants, MeOR_X1, affected carotenoid accumulation in cassava Friable Embryogenic Callus (FEC). Overexpression of maize PSY1 alone resulted in carotenoids accumulation and induced crystal formation. Co-expression with MeOR_X1 led to greatly increase of carotenoids although PSY1 expression was high in the co-expressed FEC. Our data suggest that posttranslational mechanisms controlling OR and PSY protein stability contribute to higher carotenoid levels in yellow cassava. Moreover, we showed that cassava FEC can be used to study the efficiency of single and combinatorial gene expression in increasing the carotenoid content prior to its application for the generation of biofortified cassava with enhanced carotenoids levels.
Databáze: OpenAIRE
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