Spinner-flask culture induces redifferentiation of de-differentiated chondrocytes
| Autor: | Byung Soo Kim, Hee Seok Yang, Gun-Il Im, Wan-Guen La, Tae-Jin Lee, Soo Hong Lee, Jun Yeup Seong, Suk Ho Bhang, Haeshin Lee |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Chemistry
Cartilage Type II collagen Cell Culture Techniques Bioengineering Cell Differentiation General Medicine equipment and supplies complex mixtures Applied Microbiology and Biotechnology In vitro Chondrocyte Cell biology medicine.anatomical_structure Bioreactors Chondrocytes Immunology medicine Microscopy Electron Scanning De differentiation Animals Humans Type I collagen Biotechnology |
| Zdroj: | Biotechnology letters. 33(4) |
| ISSN: | 1573-6776 |
| Popis: | Implantation of chondrocytes isolated from patients and expanded in number in vitro is being used to treat patients with cartilage injuries. However, chondrocytes de-differentiate during culture with several passages, and cartilage regenerated by implantation of de-differentiated chondrocytes may be suboptimal. Here, we show that a spinner-flask culture system induces formation of chondrocyte aggregates and redifferentiate de-differentiated chondrocytes. Spinner-flask cultures induced the aggregate formation of chondrocytes with passage 1 or 4. Importantly, spinner-flask cultures induced redifferentiation of the de-differentiated chondrocytes, as type I collagen expression was significantly lower and type II collagen expression was significantly higher in spinner flask-cultured chondrocytes than in monolayer-cultured chondrocytes. This system is easily scalable and could be feasible for clinical setting. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink